Analytical Data
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Gene name
TXNRD1
- Application
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Alternative Names
TXNRD1；GRIM12；Thioredoxin reductase 1. cytoplasmic
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q16881-5
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Expression Region
2-499aa
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AA Sequence
MASMTGGQQMGRGHHHHHHENLYFQGGEFNGPEDLPKSYDYDLIIIGGGS GGLAAAKEAAQYGK KVMVLDFVTPTPLGTRWGLGGTCVNVGCIPKKLM HQAALLGQALQDSRNYGWKVEETVKHDWDR MIEAVQNHIGSLNWGYRV ALREKKVVYENAYGQFIGPHRIKATNNKGKEKIYSAERFLIATGER PR YLGIPGDKEYCISSDDLFSLPYCPGKTLVVGASYVALECAGFLAGIGLDV TVMVRSILLRGF DQDMANKIGEHMEEHGIKFIRQFVPIKVEQIEAGTP GRLRVVAQSTNSEEIIEGEYNTVMLAIG RDACTRKIGLETVGVKINEK TGKIPVTDEEQTNVPYIYAIGDILEDKVELTPVAIQAGRLLAQR LYAG STVKCDYENVPTTVFTPLEYGACGLSEEKAVEKFGEENIEVYHSYFWPLE WTIPSRDNNK CYAKIICNTKDNERVVGFHVLGPNAGEVTQGFAAALKC GLTKKQLDSTIGIHPVCAEVFTTLSV TKRSGASILQAGCUG
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Molecular Weight
58 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TXNRD1, or Thioredoxin Reductase 1, is a vital selenoprotein that plays a critical role in cellular antioxidant defense by catalyzing the conversion of oxidized thioredoxin to its reduced form, thereby maintaining the redox balance within cells. This enzyme is particularly important in various biological processes, including the regulation of oxidative stress, cell proliferation, and apoptosis. Research has shown that dysregulation of TXNRD1 is linked to several diseases, including cancer and neurodegenerative disorders, prompting interest in its potential as a therapeutic target. Recombinant TXNRD1 protein studies enable researchers to elucidate its structural characteristics and functional mechanisms, facilitating the development of specific inhibitors or modulators that could be used in clinical applications. Additionally, understanding the effects of TXNRD1 in the context of different cellular environments contributes to our knowledge of redox biology and its implications in health and disease. The production of high-purity recombinant TXNRD1 allows for detailed kinetic assays and structural analyses, paving the way for innovative therapeutic strategies aimed at modulating its activity in pathological conditions. As such, ongoing research into TXNRD1 and its recombinant forms holds promise for advancing our understanding of cellular antioxidant mechanisms and developing novel treatment approaches for oxidative stress-related diseases.
