Analytical Data
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Gene name
PHKG2
- Application
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Alternative Names
PHKG2Phosphorylase b kinase gamma catalytic chain; liver/testis isoform; PHK-gamma-LT; PHK-gamma-T; EC 2.7.11.19; PSK-C3; Phosphorylase kinase subunit gamma-2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P15735
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Expression Region
1-406 aa
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AA Sequence
MTLDVGPEDE LPDWAAAKEF YQKYDPKDVI GRGVSSVVRR CVHRATGHEF AVKIMEVTAE RLSPEQLEEV REATRRETHI LRQVAGHPHI ITLIDSYESS SFMFLVFDLM RKGELFDYLT EKVALSEKET RSIMRSLLEA VSFLHANNIV HRDLKPENIL LDDNMQIRLS DFGFSCHLEP GEKLRELCGT PGYLAPEILK CSMDETHPGY GKEVDLWACG VILFTLLAGS PPFWHRRQIL MLRMIMEGQY QFSSPEWDDR SSTVKDLISR LLQVDPEARL TAEQALQHPF FERCEGSQPW NLTPRQRFRV AVWTVLAAGR VALSTHRVRP LTKNALLRDP YALRSVRHLI DNCAFRLYGH WVKKGEQQNR AALFQHRPPG PFPIMGPEEE GDSAAITEDE AVLVLG
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Molecular Weight
46.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PHKG2 (phosphorylase kinase gamma 2) is a critical enzyme involved in glycogen metabolism, primarily regulating glycogenolysis—the breakdown of glycogen to glucose. Mutations in the PHKG2 gene have been linked to various metabolic disorders, particularly those affecting muscle function and glucose homeostasis, such as exercising intolerance, myopathy, and hypoglycemic episodes. The significance of studying PHKG2 lies in its role in muscle and liver tissues, where it ensures proper energy mobilization during periods of increased energy demand. Research on PHKG2 recombinant proteins has gained momentum due to the enzyme's potential as a therapeutic target for treating glycogen storage diseases and related metabolic syndromes. Recombinant production allows for detailed investigations into the enzyme's structure, function, and regulatory mechanisms, providing insights that could lead to novel pharmacological interventions. Furthermore, understanding PHKG2's biochemical pathways could illuminate broader aspects of metabolic regulation, contributing to strategies for managing obesity and diabetes. Thus, the study of PHKG2 recombinant proteins is not only crucial for comprehending glycogen metabolism but also holds promise for developing effective treatments for metabolic disorders.
