Analytical Data
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Gene name
YBX1
- Application
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Alternative Names
YBX1；NSEP1；Y-box-binding Protein 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P67809
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Expression Region
1-324aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSSEAET QQPPAAPPAA PALSAADTKP GTTGSGAGSG GPGGLTSAAP AGGDKKVIAT KVLGTVKWFN VRNGYGFINR NDTKEDVFVH QTAIKKNNPR KYLRSVGDGE TVEFDVVEGE KGAEAANVTG PGGVPVQGSK YAADRNHYRR YPRRRGPPRN YQQNYQNSES GEKNEGSESA PEGQAQQRRP YRRRRFPPYY MRRPYGRRPQ YSNPPVQGEV MEGADNQGAG EQGRPVRQNM YRGYRPRFRR GPPRQRQPRE DGNEEDKENQ GDETQGQQPP QRRYRRNFNY RRRRPENPKP QDGKETKAAD PPAENSSAPE AEQGGAE
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Molecular Weight
38 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
YBX1, or Y-box binding protein 1, is a crucial RNA-binding protein that plays a significant role in various cellular processes, including transcription regulation, mRNA splicing, and translation initiation. In recent years, research has demonstrated that YBX1 is involved in multiple biological functions, such as cell proliferation, differentiation, and apoptosis, making it a critical factor in cancer development and progression. Elevated levels of YBX1 have been associated with several types of cancer, where it often acts as an oncogene by stabilizing oncogenic mRNAs and inhibiting the expression of tumor suppressor genes. Additionally, YBX1 is implicated in stress response mechanisms, especially under conditions like oxidative stress and hypoxia, where it regulates the expression of genes involved in cellular survival and adaptation. The study of recombinant YBX1 protein allows for detailed investigations into its structural and functional characteristics, facilitating the understanding of its molecular interactions and pathways. This research offers insights that could lead to the development of potential therapeutic strategies targeting YBX1 in cancer treatment, given its role in tumor biology and response to therapy. Ultimately, the elucidation of YBX1's functions at the molecular level holds promise for advancing our knowledge of cancer mechanisms and identifying novel biomarkers for diagnosis and prognosis.
