Analytical Data
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Gene name
PIGZ
- Application
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Alternative Names
PIGZ; SMP3; GPI mannosyltransferase 4; EC 2.4.1.-; GPI mannosyltransferase IV; GPI-MT-IV; Phosphatidylinositol-glycan biosynthesis class Z protein; PIG-Z; SMP3 homolog; hSMP3
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q86VD9
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Expression Region
1-579 aa
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AA Sequence
MQICGSSVASVAAGTSFQVLGPVCWQQLDLKMAVRVLWGGLSLLRVLWCLLPQTGYVHPDEFFQSPEVMAEDILGVQAARPWEFYPSSSCRSVLFPLLISGSTFWLLRLWEELGPWPGLVSGYALLVGPRLLLTALSFALDGAVYHLAPPMGADRWNALALLSGSYVTLVFYTRTFSNTIEGLLFTWLLVLVSSHVTWGPTRKEPAPGPRWRSWLLGGIVAAGFFNRPTFLAFAVVPLYLWGTRGATNPGLKSLTREALVLLPGATLTAAVFVATDSWYFSSPATSRNLVLTPVNFLHYNLNPQNLARHGTHARLTHLAVNGFLLFGVLHAQALQAAWQQLQVGLQASAQMGLLRALGARSLLSSPRSYLLLLYFMPLALLSAFSHQEARFLIPLLVPLVLLCSPQTQPVPWKGTVVLFNALGALLFGCLHQGGLVPGLEYLEQVVHAPVLPSTPTHYTLLFTHTYMPPRHLLHLPGLGAPVEVVDIGGTEDWALCQTLKSFTRQPACQVAGGPWLCRLFVVTPGTTRRAVEKCSFPFKNETLLFPHLTLEDPPALSSLLSGAWRDHLSLHIVELGEET
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Molecular Weight
89.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PIGZ (Phosphatidylinositol glycan anchor biosynthesis class Z) is a critical enzyme involved in the post-translational modification of proteins, specifically in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors, which play an essential role in anchoring proteins to cell membranes. Research has highlighted the significance of PIGZ in various biological processes, including cell signaling, adhesion, and immune responses. Mutations or dysregulation of PIGZ can lead to various diseases, including certain types of cancer and developmental disorders. The study of PIGZ-recombinant proteins has gained momentum as it provides insights into the enzyme's structure-function relationship and its interactions with other cellular components. By employing advanced techniques such as recombinant DNA technology, researchers can produce PIGZ proteins for biochemical characterization, high-throughput screening, and potential therapeutic applications. Understanding the functional mechanisms of PIGZ not only enhances our knowledge of GPI-anchor biosynthesis but also paves the way for novel therapeutic strategies targeting GPI-anchored proteins in human diseases. Overall, PIGZ represents a promising area of study within the broader field of cell biology and molecular medicine, emphasizing the intricate connections between protein modifications and cellular functionality.
