Analytical Data
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Gene name
BBS4
- Application
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Alternative Names
Bardet Biedl syndrome 4 Protein; Bardet-Biedl syndrome 4 Protein; Bbs4; BBS4_HUMAN
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96RK4
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Expression Region
1-519aa
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AA Sequence
MAEERVATRT QFPVSTESQK PRQKKAPEFP ILEKQNWLIH LHYIRKDYEA CKAVIKEQLQ ETQGLCEYAI YVQALIFRLE GNIQESLELF QTCAVLSPQS ADNLKQVARS LFLLGKHKAA IEVYNEAAKL NQKDWEISHN LGVCYIYLKQ FNKAQDQLHN ALNLNRHDLT YIMLGKIHLL EGDLDKAIEV YKKAVEFSPE NTELLTTLGL LYLQLGIYQK AFEHLGNALT YDPTNYKAIL AAGSMMQTHG DFDVALTKYR VVACAVPESP PLWNNIGMCF FGKKKYVAAI SCLKRANYLA PFDWKILYNL GLVHLTMQQY ASAFHFLSAA INFQPKMGEL YMLLAVALTN LEDIENAKRA YAEAVHLDKC NPLVNLNYAV LLYNQGEKKN ALAQYQEMEK KVSLLKDNSS LEFDSEMVEM AQKLGAALQV GEALVWTKPV KDPKSKHQTT STSKPASFQQ PLGSNQALGQ AMSSAAAYRT LPSGAGGTSQ FTKPPSLPLE PEPAVESSPT ETSEQIREK
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Molecular Weight
82.83 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
BBS4, or Bardet-Biedl syndrome 4 protein, is a member of a group of proteins implicated in the complex pathology of Bardet-Biedl syndrome (BBS), a rare genetic disorder characterized by a range of symptoms including obesity, retinal degeneration, polydactyly, and renal anomalies. The study of BBS4 and its structural and functional properties is crucial in understanding the molecular mechanisms underlying BBS. Recent research has highlighted the importance of BBS4 in ciliary function and cell signaling, as it plays a pivotal role in the assembly of the BBSome, a protein complex essential for the proper functioning of cilia. Genetic mutations in BBS4 disrupt these processes, leading to the diverse clinical manifestations observed in affected individuals. By exploring the recombinant expression and purification of BBS4, researchers aim to elucidate its function, study its interactions with other BBS proteins, and investigate the consequences of specific mutations. Such studies not only improve our understanding of the pathophysiology of BBS but also may pave the way for potential therapeutic strategies aimed at mitigating the symptoms of this syndrome. The analysis of BBS4 in a biological context could also provide insights into broader cellular mechanisms relevant to cilia-related diseases, positioning it as a significant focus of biomedical research.
