Analytical Data
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Gene name
POLD3
- Application
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Alternative Names
DNA polymerase delta subunit 3; DNA polymerase delta subunit p66; DNA polymerase delta; subunit 3; DNA polymerase subunit delta p66; DPOD3_HUMAN; KIAA0039; P66; P68; Pol delta C subunit (p66); Pold3; Polymerase (DNA directed); delta 3; Polymerase (DNA-directed); delta 3; accessory subunit; PPP1R128; Protein phosphatase 1; regulatory subunit 128
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q15054
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Expression Region
2-466 aa
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AA Sequence
ADQLYLENI DEFVTDQNKI VTYKWLSYTL GVHVNQAKQM LYDYVERKRK ENSGAQLHVT YLVSGSLIQN GHSCHKVAVV REDKLEAVKS KLAVTASIHV YSIQKAMLKD SGPLFNTDYD ILKSNLQNCS KFSAIQCAAA VPRAPAESSS SSKKFEQSHL HMSSETQANN ELTTNGHGPP ASKQVSQQPK GIMGMFASKA AAKTQETNKE TKTEAKEVTN ASAAGNKAPG KGNMMSNFFG KAAMNKFKVN LDSEQAVKEE KIVEQPTVSV TEPKLATPAG LKKSSKKAEP VKVLQKEKKR GKRVALSDDE TKETENMRKK RRRIKLPESD SSEDEVFPDS PGAYEAESPS PPPPPSPPLE PVPKTEPEPP SVKSSSGENK RKRKRVLKSK TYLDGEGCIV TEKVYESESC TDSEEELNMK TSSVHRPPAM TVKKEPREER KGPKKGTAAL GKANRQVSIT GFFQRK
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Molecular Weight
51.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
POLD3, or DNA polymerase delta 3, is a critical enzyme involved in DNA replication and repair processes. As a part of the DNA polymerase delta complex, POLD3 plays a significant role in maintaining genomic stability by facilitating accurate DNA synthesis and proofreading. Research into the POLD3 recombinant protein has gained momentum due to its implications in cancer biology, where mutations or dysregulation of DNA polymerases can lead to genomic instability and tumorigenesis. Additionally, POLD3 is essential for various cellular processes such as cell cycle regulation and response to DNA damage, making it a vital target for understanding the mechanisms of DNA repair pathways. Studies have indicated that alterations in POLD3 expression or function can contribute to cellular resistance to chemotherapeutic agents, highlighting its potential as a biomarker for cancer prognosis and as a therapeutic target. The recombinant expression of POLD3 allows for detailed structural and functional analyses, facilitating investigations into its enzymatic activity, interactions with other proteins, and role in cellular response to DNA damage. Given its essential functions, understanding the biology of POLD3 and its recombinant forms can provide insights into both fundamental cellular processes and the development of cancer therapeutics. Thus, ongoing research in this area aims to elucidate the precise mechanisms by which POLD3 contributes to DNA fidelity and repair, with the goal of developing innovative strategies for cancer treatment and prevention.
