Analytical Data
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Gene name
VPS35
- Application
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Alternative Names
VPS35；MEM3；Vacuolar Protein sorting-associated Protein 35
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96QK1
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Expression Region
1-796aa
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AA Sequence
MPTTQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNML GELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQY AGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLR NYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQ GHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQV VNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIII ALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSL QVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTR LLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTE IVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSLVGRFIHLLRS EDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVD DKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHE TVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLR TQCALAASKLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECL KKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKI REDLPNLESSEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLIL
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Molecular Weight
118 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
VPS35 (Vacuolar Protein Sorting 35) is a crucial component of the retromer complex, which plays a significant role in the intracellular transport and recycling of proteins within cells. Recent studies have highlighted the importance of VPS35 in maintaining cellular homeostasis and its implications in various neurodegenerative disorders, particularly Parkinson's disease. Mutations in the VPS35 gene have been associated with familial forms of Parkinson's, suggesting that disruptions in its function can lead to impaired neuronal survival and the accumulation of toxic protein aggregates. The study of VPS35 and its role in endosomal trafficking provides valuable insights into the mechanisms underlying protein misfolding diseases. Researchers have focused on understanding the structure-function relationship of VPS35 and its interactions with other retromer components, aiming to elucidate its contribution to the overall function of the retromer complex. By examining VPS35 in the context of cellular and molecular biology, scientists hope to uncover potential therapeutic targets for restoring normal protein transport mechanisms and developing strategies to combat neurodegenerative conditions linked to VPS35 dysfunction. The ongoing exploration of VPS35 and its reconstituted protein variants not only enhances our understanding of fundamental cellular processes but also paves the way for innovative approaches to treat diseases challenging modern medicine.
