Analytical Data
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Gene name
BTRC
- Application
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Alternative Names
Beta transducin repeat containing E3 ubiquitin Protein ligase; beta TrCP1; beta-transducin repeat containing ; BETA-TRCP; betaTrCP; btrC; bTrCP; bTrCP1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9Y297
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Expression Region
1-605aa
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AA Sequence
MDPAEAVLQE KALKFMCSMP RSLWLGCSSL ADSMPSLRCL YNPGTGALTA FQNSSEREDC NNGEPPRKII PEKNSLRQTY NSCARLCLNQ ETVCLASTAM KTENCVAKTK LANGTSSMIV PKQRKLSASY EKEKELCVKY FEQWSESDQV EFVEHLISQM CHYQHGHINS YLKPMLQRDF ITALPARGLD HIAENILSYL DAKSLCAAEL VCKEWYRVTS DGMLWKKLIE RMVRTDSLWR GLAERRGWGQ YLFKNKPPDG NAPPNSFYRA LYPKIIQDIE TIESNWRCGR HSLQRIHCRS ETSKGVYCLQ YDDQKIVSGL RDNTIKIWDK NTLECKRILT GHTGSVLCLQ YDERVIITGS SDSTVRVWDV NTGEMLNTLI HHCEAVLHLR FNNGMMVTCS KDRSIAVWDM ASPTDITLRR VLVGHRAAVN VVDFDDKYIV SASGDRTIKV WNTSTCEFVR TLNGHKRGIA CLQYRDRLVV SGSSDNTIRL WDIECGACLR VLEGHEELVR CIRFDNKRIV SGAYDGKIKV WDLVAALDPR APAGTLCLRT LVEHSGRVFR LQFDEFQIVS SSHDDTILIW DFLNDPAAQA EPPRSPSRTY TYISR
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Molecular Weight
68.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
BTRC (Beta-transducin repeat containing E3 ubiquitin protein ligase) is an essential component of the ubiquitin-proteasome system, which regulates various cellular processes by targeting specific proteins for degradation. This protein plays a crucial role in modulating several signaling pathways, including those related to cell cycle regulation, transcriptional control, and immune responses. Research on BTRC has gained significant attention due to its involvement in numerous diseases, particularly cancer, where dysregulation of ubiquitination processes can lead to uncontrolled cell proliferation and tumor progression. Moreover, BTRC has been identified as a potential therapeutic target, as its modulation may enhance the efficacy of existing cancer treatments or contribute to novel therapeutic strategies. The study of BTRC recombinant proteins has allowed researchers to investigate its functional properties, interaction with other proteins, and regulatory mechanisms more precisely. By producing BTRC in a recombinant form, scientists can perform high-throughput assays and structural studies, aiding in the understanding of its role in cellular pathways and disease states. Furthermore, investigating the structural and functional aspects of BTRC can lead to the identification of small molecules or peptides that can modulate its activity, presenting new opportunities for targeted therapy. Overall, the research background surrounding BTRC recombinant proteins underlines their significance in cellular metabolism and the potential for advancing therapeutic interventions in diseases driven by ubiquitin system dysfunctions.
