Analytical Data
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Gene name
POLH
- Application
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Alternative Names
DKFZp781A0112; DNA polymerase eta; DNA polymerase theta; DPOLQ_HUMAN; POLH; POLQ; Polymerase (DNA directed) theta; PRO0327
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O75417
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Expression Region
1-414 aa
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AA Sequence
MATGQDRVVALVDMDCFFVQVEQRQNPHLRNKPCAVVQYKSWKGGGIIAVSYEARAFGVTRSMWADDAKKLCPDLLLAQVRESRGKANLTKYREASVEVMEIMSRFAVIERASIDEAYVDLTSAVQERLQKLQGQPISADLLPSTYIEGLPQGPTTAEETVQKEGMRKQGLFQWLDSLQIDNLTSPDLQLTVGAVIVEEMRAAIERETGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLFSQMPIRKIRSLGGKLGASVIEILGIEYMGELTQFTESQLQSHFGEKNGSWLYAMCRGIEHDPVKPRQLPKTIGCSKNFPGKTALATREQVQWWLLQLAQELEERLTKDRNDNDRVATQLVVSIRVQGDKRLSSLRRCCALTRYDAHKMSHDAFTVIKNCNTSGIQTE
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Molecular Weight
71.28 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
POLH (Polymerase Eta) is a specialized DNA polymerase involved in translesion synthesis, a mechanism that allows cells to replicate DNA even when encountering damaged bases, such as those produced by ultraviolet (UV) light. POLH plays a crucial role in maintaining genomic stability, as it helps bypass lesions that could otherwise lead to replication fork stalling and subsequent cellular dysfunction. Mutations in the POLH gene are associated with a rare genetic disorder known as Xeroderma Pigmentosum variant (XP-V), which increases susceptibility to skin cancer due to impaired ability to repair UV-induced DNA damage. Research into POLH recombinant proteins provides insights into its structure, function, and interaction with DNA and other cellular partners. Understanding the biochemical properties of POLH, including its fidelity and efficiency in incorporating nucleotides opposite damaged bases, can elucidate its role in cellular defense mechanisms against genotoxic stress. Furthermore, studying POLH can inform therapeutic strategies aimed at enhancing DNA damage tolerance in cancer cells, potentially leading to improved outcomes in cancer treatment by targeting repair pathways and customizing chemotherapeutic interventions.
