Analytical Data
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基因名
SGSH
- Application
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别名
SGSH；HSS；N-sulphoglucosamine sulphohydrolase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P51688
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表达区间
1-502aa
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氨基酸序列
MSCPVPACCALLLVLGLCRARPRNALLLLADDGGFESGAYNNSAIATPHL DALARRSLLFRNAFTSVSSCSPSRASLLTGLPQHQNGMYGLHQDVHHFNS FDKVRSLPLLLSQAGVRTGIIGKKHVGPETVYPFDFAYTEENGSVLQVGR NITRIKLLVRKFLQTQDDRPFFLYVAFHDPHRCGHSQPQYGTFCEKFGNG ESGMGRIPDWTPQAYDPLDVLVPYFVPNTPAARADLAAQYTTVGRMDQGV GLVLQELRDAGVLNDTLVIFTSDNGIPFPSGRTNLYWPGTAEPLLVSSPE HPKRWGQVSEAYVSLLDLTPTILDWFSIPYPSYAIFGSKTIHLTGRSLLP ALEAEPLWATVFGSQSHHEVTMSYPMRSVQHRHFRLVHNLNFKMPFPIDQ DFYVSPTFQDLLNRTTAGQPTGWYKDLRHYYYRARWELYDRSRDPHETQN LATDPRFAQLLEMLRDQLAKWQWETHDPWVCAPDGVLEEKLSPQCQPLHN EL
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分子量
81 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of SGSH (N-sulfoglucosamine sulfohydrolase) recombinant proteins is pivotal in understanding the biochemical pathways involved in heparan sulfate metabolism and the etiology of related lysosomal storage disorders, particularly mucopolysaccharidosis type IIIA (MPS IIIA). MPS IIIA is caused by mutations in the SGSH gene, leading to enzymatic deficiency and subsequent accumulation of glycosaminoglycans in various tissues. This research is essential not only for elucidating the molecular mechanisms underlying the disorder but also for developing potential therapeutic strategies, including enzyme replacement therapy and gene therapy. By expressing SGSH as a recombinant protein, researchers can investigate its structure, function, and interactions with substrates and inhibitors, ultimately aiming to identify effective treatments that can alleviate the symptoms of MPS IIIA. The advancement of recombinant protein technology facilitates the production of SGSH in sufficient quantities for biochemical assays and functional studies, providing insights that could lead to breakthroughs in managing this debilitating condition. Furthermore, studying the dynamics of SGSH and its variants contributes to a broader understanding of lysosomal functions, paving the way for innovations in the treatment of various lysosomal storage diseases.
