Analytical Data
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Gene name
RNF212
- Application
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Alternative Names
RNF212；Probable E3 SUMO-Protein ligase RNF212
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q495C1
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Expression Region
1-297aa
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AA Sequence
MANWVFCNRCFQPPHRTSCFSLTNCGHVYCDACLGKGKKNECLICKAPCRTVLLSKHTDADIQAFFMSIDSLCKKYSRETSQILEFQEKHRKRLLAFYREKISRLEESLRKSVLQIEQLQSMRSSQQTAFSTIKSSVSTKPHGCLLPPHSSAPDRLESMEVDLSPSPIRKSEIAAGPARISMISPPQDGRMGPHLTASFCFIPWLTLSKPPVPGECVISRGSPCFCIDVCPHWLLLLAFSSGRHGELTNSKTLPIYAEVQRAVLFPFQQAEGTLDTFRTPAVSVVFPLCQFERKKSF
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Molecular Weight
60.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
RNF212, a member of the RBR E3 ubiquitin ligase family, has garnered significant attention in recent years due to its essential role in meiosis and genomic stability. It is primarily involved in the regulation of homologous recombination during gametogenesis, where it facilitates the proper assembly and disassembly of the synaptonemal complex, a critical structure that holds homologous chromosomes together. Mutations in RNF212 have been linked to reproductive disorders and chromosomal abnormalities, indicating its importance in fertility and genomic integrity. Research on recombinant RNF212 protein has focused on elucidating its functional mechanisms, interaction partners, and its regulatory role in both meiosis and mitotic processes. The production of recombinant RNF212 allows for biochemical assays that can dissect its ubiquitination activity and investigate its potential as a therapeutic target in treating infertile conditions or other disorders linked to DNA repair deficiencies. Overall, understanding RNF212's function at a molecular level is crucial for advancing our knowledge of meiosis, as well as its implications in human health and disease.
