Analytical Data
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基因名
RASGEF1B
- Application
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别名
FLJ31695; GPI gamma-4; GPIG4; MGC46251; Ras-GEF domain-containing family member 1B; RasGEF domain family member 1B; RASGEF1B; RGF1B_HUMAN
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q0VAM2
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表达区间
1-473 aa
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氨基酸序列
MPQTPPFSAM FDSSGYNRNL YQSAEDSCGG LYYHDNNLLS GSLEALIQHL VPNVDYYPDR TYIFTFLLSS RLFMHPYELM AKVCHLCVEH QRLSDPDSDK NQMRKIAPKI LQLLTEWTET FPYDFRDERM MRNLKDLAHR IASGEEQTYR KNVQQMMQCL IRKLAALSQY EEVLAKISST STDRLTVLKT KPQSIQRDII TVCNDPYTLA QQLTHIELER LNYIGPEEFV QAFVQKDPLD NDKSCYSERK KTRNLEAYVE WFNRLSYLVA TEICMPVKKK HRARMIEYFI DVARECFNIG NFNSLMAIIS GMNMSPVSRL KKTWAKVKTA KFDILEHQMD PSSNFYNYRT ALRGAAQRSL TAHSSREKIV IPFFSLLIKD IYFLNEGCAN RLPNGHVNFE KFWELAKQVS EFMTWKQVEC PFERDRKILQ YLLTVPVFSE DALYLASYES EGPENHIEKD RWKSLRSSLL GRV
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分子量
55.3 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RASGEF1B, a member of the RAS guanine nucleotide exchange factor (GEF) family, plays a critical role in cellular signaling by facilitating the activation of RAS proteins, which are pivotal in regulating various cellular processes such as growth, differentiation, and survival. Research into RASGEF1B has gained momentum due to its potential implications in cancer and other diseases characterized by aberrant RAS signaling. Understanding the molecular mechanisms by which RASGEF1B activates RAS proteins can provide insights into the intricacies of RAS-related pathways and their contributions to tumorigenesis. Studies have indicated that RASGEF1B not only directly interacts with RAS, but also engages with other signaling modulators, indicating a more complex regulatory role in the signaling network. The recombinant expression of RASGEF1B provides a powerful tool to investigate its biochemical properties, enzymatic activity, and interactions with other proteins, thereby elucidating its functional significance in health and disease. Insights gained from RASGEF1B research could ultimately inform therapeutic strategies aimed at modulating RAS signaling in pathological conditions.
