Analytical Data
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基因名
NAGLU
- Application
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别名
NAGLU；UFHSD1；Alpha-N-acetylglucosaminidase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P54802
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表达区间
24-743aa
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氨基酸序列
DEAREAA AVRALVARLL GPGPAADFSV SVERALAAKP GLDTYSLGGG GAARVRVRGS TGVAAAAGLH RYLRDFCGCH VAWSGSQLRL PRPLPAVPGE LTEATPNRYR YYQNVCTQSY SFVWWDWARW EREIDWMALN GINLALAWSG QEAIWQRVYL ALGLTQAEIN EFFTGPAFLA WGRMGNLHTW DGPLPPSWHI KQLYLQHRVL DQMRSFGMTP VLPAFAGHVP EAVTRVFPQV NVTKMGSWGH FNCSYSCSFL LAPEDPIFPI IGSLFLRELI KEFGTDHIYG ADTFNEMQPP SSEPSYLAAA TTAVYEAMTA VDTEAVWLLQ GWLFQHQPQF WGPAQIRAVL GAVPRGRLLV LDLFAESQPV YTRTASFQGQ PFIWCMLHNF GGNHGLFGAL EAVNGGPEAA RLFPNSTMVG TGMAPEGISQ NEVVYSLMAE LGWRKDPVPD LAAWVTSFAA RRYGVSHPDA GAAWRLLLRS VYNCSGEACR GHNRSPLVRR PSLQMNTSIW YNRSDVFEAW RLLLTSAPSL ATSPAFRYDL LDLTRQAVQE LVSLYYEEAR SAYLSKELAS LLRAGGVLAY ELLPALDEVL ASDSRFLLGS WLEQARAAAV SEAEADFYEQ NSRYQLTLWG PEGNILDYAN KQLAGLVANY YTPRWRLFLE ALVDSVAQGI PFQQHQFDKN VFQLEQAFVL SKQRYPSQPR GDTVDLAKKI FLKYYPRWVA GSW
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NAGLU (N-acetyl-alpha-glucosaminidase) is an essential lysosomal enzyme responsible for the breakdown of glycosaminoglycans, particularly heparin and heparan sulfate. Deficiencies in NAGLU activity lead to the lysosomal storage disorder known as Sanfilippo syndrome type B, a condition characterized by progressive neurodegeneration, cognitive decline, and severe behavioral issues, which significantly impact the quality of life. The study of recombinant NAGLU has gained prominence as researchers aim to develop effective enzyme replacement therapies to address the enzymatic deficit in affected individuals. By employing various recombinant DNA technologies, scientists can produce large quantities of functional NAGLU protein, facilitating in-depth studies into its enzymatic properties, stability, and therapeutic potential. Furthermore, understanding the structure and function of this enzyme is critical for the design of pharmacological chaperones that can enhance its activity and improve the substrate accessibility in lysosomes. The ongoing research on NAGLU is also vital for investigating gene therapy approaches, wherein the aim is to supply the missing or faulty enzyme to cells, thus alleviating the metabolic burden associated with its deficiency. Overall, the exploration of recombinant NAGLU represents a crucial step toward advancing treatment options for Sanfilippo syndrome type B and similar lysosomal storage disorders, reflecting the broader effort to address genetic diseases caused by enzyme deficiencies.
