Analytical Data
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Gene name
ST8SIA2
- Application
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Alternative Names
ST8SIA2; SIAT8B; STX; Alpha-2.8-sialyltransferase 8B; EC 2.4.99.-; Sialyltransferase 8B; SIAT8-B; Sialyltransferase St8Sia II; ST8SiaII; Sialyltransferase X; STX
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q92186
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Expression Region
1-354 aa
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AA Sequence
MQLQFRSWMLAALTLLVVFLIFADISEIEEEIGAEVVINGSSSPAVVDRSNESIKHNIQPASSKWRHNQTLSLRIRKQILKFSDAEKDISVLKGTLKPGDIIHYIFDRDSTMNVSQNLYELLPRTSPLKNKHFGTCAIVGNSGVLLNSGCGQEIDAHSFVIRCNLAPVQEYARDVGLKTDLVTMNPSVIQRAFEDLVNATWREKLLQRLHSLNGSILWIPAFMARGGKERVEWVNELILKHHVNVRTAYPSLRLLHAVRGYWLTNKVHIKRPTTGLLMYTLATRFCKQIYLYGFWPFPLDQNQNPVKYHYYDSLKYGYTSQASPHTMPLEFKALKSLHEQGALKLTVGQCDGAT
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Molecular Weight
66.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ST8SIA2, a member of the ST8Sia family of sialyltransferases, plays a critical role in the synthesis of polysialic acid (polySia), which is crucial for various physiological processes, including neuronal development and brain plasticity. Research has shown that ST8SIA2 is involved in the modulation of cell signaling, cell-cell interactions, and has implications in neurodevelopmental disorders and tumors. The enzyme catalyzes the transfer of sialic acid residues to glycoproteins and glycolipids, influencing the structural properties of the glycocalyx on cell surfaces. Dysregulation of ST8SIA2 has been linked to several pathological conditions, making it a target of interest for therapeutic interventions. Current studies focus on understanding its biochemical mechanisms, regulation, and potential as a biomarker or therapeutic target in diseases characterized by aberrant sialylation. The recombinant expression of ST8SIA2 enables in-depth characterization of its enzymatic activity, substrate specificity, and interactions, providing insights into its functional roles in health and disease. As sialic acids significantly impact cellular behavior, unraveling the complexities of ST8SIA2 holds promise for the development of novel strategies in regenerative medicine and cancer therapy.
