Analytical Data
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Gene name
F2R
- Application
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Alternative Names
F2R; CF2R; PAR1; TR; Proteinase-activated receptor 1; PAR-1; Coagulation factor II receptor; Thrombin receptor
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P25116
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Expression Region
1-425aa
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AA Sequence
MGPRRLLLVAACFSLCGPLLSARTRARRPESKATNATLDPRSFLLRNPNDKYEPFWEDEEKNESGLTEYRLVSINKSSPLQKQLPAFISEDASGYLTSSWLTLFVPSVYTGVFVVSLPLNIMAIVVFILKMKVKKPAVVYMLHLATADVLFVSVLPFKISYYFSGSDWQFGSELCRFVTAAFYCNMYASILLMTVISIDRFLAVVYPMQSLSWRTLGRASFTCLAIWALAIAGVVPLLLKEQTIQVPGLNITTCHDVLNETLLEGYYAYYFSAFSAVFFFVPLIISTVCYVSIIRCLSSSAVANRSKKSRALFLSAAVFCIFIICFGPTNVLLIAHYSFLSHTSTTEAAYFAYLLCVCVSSISCCIDPLIYYYASSECQRYVYSILCCKESSDPSSYNSSGQLMASKMDTCSSNLNNSIYKKLLT
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Molecular Weight
47.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
F2R, also known as the coagulation factor II receptor or thrombin receptor, is a G protein-coupled receptor (GPCR) that plays a crucial role in various physiological processes, including hemostasis, inflammation, and cell proliferation. It is activated by the serine protease thrombin, which cleaves the receptor and initiates intracellular signaling pathways that lead to diverse biological effects. Due to its involvement in critical pathways, F2R has garnered interest as a potential therapeutic target for various conditions, including thrombosis, cancer, and inflammatory diseases. Researchers are focusing on producing recombinant F2R proteins to better understand its structure-function relationships and the mechanisms underlying its signaling. The generation of F2R recombinant proteins allows for detailed studies using techniques such as crystallography and biophysical characterization, facilitating the identification of small molecules or antibodies that can modulate its activity. Additionally, the investigation of F2R's role in different cellular contexts and diseases is crucial for the development of targeted therapies. Given its central role in multiple pathophysiological conditions, F2R stands as a promising candidate for drug development, highlighting the significance of recombinant protein studies in translating fundamental research into clinical applications. Overall, the study of F2R recombinant proteins not only enhances our understanding of thrombin signaling but also paves the way for innovative treatments aimed at mitigating the impact of related diseases.
