Analytical Data
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基因名
TOP2A
- Application
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别名
alpha isozyme; ATP hydrolyzing DNA topoisomerase II alfa; DNA gyrase; DNA topoisomerase (ATP hydrolyzing); DNA topoisomerase 2 alpha; DNA topoisomerase 2-alpha; DNA topoisomerase II 170 kD; DNA topoisomerase II alpha isozyme; DNA topoisomerase II; DNA Topoisomerase2; TOP 2A; TOP2; TOP2A; TOP2A_HUMAN; Topoisomerase DNA II alpha 170kDa; TP2A
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P11388
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表达区间
1435-1531 aa
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氨基酸序列
RAAPKGTKRDPALNSGVSQKPDPAKTKNRRKRKPSTSDDSDSNFEKIVSKAVTSKKSKGESDDFHMDFDSAVAPRAKSVRAKKPIKYLEESDEDDLF
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分子量
36.41 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TOP2A, or Topoisomerase II Alpha, is a crucial enzyme that plays a significant role in DNA replication, repair, and chromatin organization. It is essential for resolving DNA supercoiling and tangling during cell division, thus ensuring proper segregation of chromosomes. Abnormal expression or activity of TOP2A has been implicated in various cancers, making it a potential therapeutic target. Recent studies have investigated TOP2A as a biomarker for predicting treatment responses in cancers, particularly in combination therapies involving chemotherapeutic agents. The enzyme's dual role in maintaining genomic stability while also being a target for DNA-damaging agents has sparked intense research into its structure and function. Given its importance in mitotic and meiotic processes, scientists have focused on characterizing recombinant TOP2A proteins to elucidate their structural and functional properties. This has led to advances in understanding how mutations or post-translational modifications affect its activity, ultimately informing drug development strategies aimed at enhancing the effectiveness of cancer therapies. As researchers continue to explore the intricacies of TOP2A, its potential as a target for targeted therapies remains a significant area of focus, particularly in tailoring personalized medicine approaches for patients with TOP2A-related malignancies. Overall, the study of recombinant TOP2A proteins promises to deepen our understanding of cellular processes and pave the way for innovative therapeutic interventions in cancer treatment.
