Analytical Data
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Gene name
TTLL10
- Application
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Alternative Names
TTLL10; Inactive polyglycylase TTLL10; Tubulin--tyrosine ligase-like Protein 10
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q6ZVT0
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Expression Region
1-673 aa
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AA Sequence
MDHSCTRFIH RRGPPTRTRA GFKRGKRPRI QQRPRARVSG TIPASRLHPA PASQPGPCPA PGHCPVGPAH ERPMGSSQEE GLRCQPSQPD HDADGHCGPD LEGAERASAT PGPPGLLNSH RPADSDDTNA AGPSAALLEG LLLGGGKPSP HSTRPGPFFY IGGSNGATII SSYCKSKGWQ RIHDSRRDDY TLKWCEVKSR DSYGSFREGE QLLYQLPNNK LLTTKIGLLS TLRGRARAMS KASKVPGGVQ ARLEKDAAAP ALEDLPWTSP GYLRPQRVLR MEEFFPETYR LDLKHEREAF FTLFDETQIW ICKPTASNQG KGIFLLRNQE EVAALQAKTR SMEDDPIHHK TPFRGPQARV VQRYIQNPLL VDGRKFDVRS YLLIACTTPY MIFFGHGYAR LTLSLYDPHS SDLGGHLTNQ FMQKKSPLYM LLKEHTVWSM EHLNRYISDT FWKARGLAKD WVFTTLKKRM QQIMAHCFLA AKPKLDCKLG YFDLIGCDFL IDDNFKVWLL EMNSNPALHT NCEVLKEVIP GVVIETLDLV LETFRKSLRG QKMLPLLSQR RFVLLHNGEA DPRPHLGGSC SLRRWPPLPT RQAKSSGPPM PHAPDQPGAR RPAPPPLVPQ RPRPPGPDLD SAHDGEPQAP GTEQSGTGNR HPAQEPSPGT AKEEREEPEN ARP
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Molecular Weight
75.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
TTLL10, a member of the tubulin tyrosine ligase-like (TTLL) family, plays a critical role in the post-translational modification of tubulin, specifically in the addition of tyrosine residues to the carboxyl terminus. This modification is essential for the regulation of microtubule dynamics and stability, impacting various cellular processes, including cell division, intracellular transport, and signaling pathways. Recent studies have suggested that TTLL10 may be involved in cancer progression and neurodegenerative diseases, highlighting its potential as a therapeutic target. The understanding of TTLL10's structure and function is still evolving, but its significance in microtubule regulation and the broader implication in health and disease makes it a compelling subject for research. Investigations into TTLL10's reconstitution, enzymatic mechanisms, and interaction with other cellular components are crucial for unveiling its biological roles and developing novel strategies for disease intervention.
