Analytical Data
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Gene name
USP22
- Application
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Alternative Names
Deubiquitinating enzyme 22; KIAA1063; Ubiquitin carboxyl terminal hydrolase 22; Ubiquitin carboxyl-terminal hydrolase 22; Ubiquitin specific peptidase 22; Ubiquitin specific peptidase 3 like; Ubiquitin specific processing protease 22; Ubiquitin specific protease 22; Ubiquitin thioesterase 22; Ubiquitin thiolesterase 22; Ubiquitin-specific-processing protease 22; UBP22_HUMAN; USP 22; Usp22; USP3L
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9UPT9
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Expression Region
1-525 aa
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AA Sequence
MVSRPEPEGE AMDAELAVAP PGCSHLGSFK VDNWKQNLRA IYQCFVWSGT AEARKRKAKS CICHVCGVHL NRLHSCLYCV FFGCFTKKHI HEHAKAKRHN LAIDLMYGGI YCFLCQDYIY DKDMEIIAKE EQRKAWKMQG VGEKFSTWEP TKRELELLKH NPKRRKITSN CTIGLRGLIN LGNTCFMNCI VQALTHTPLL RDFFLSDRHR CEMQSPSSCL VCEMSSLFQE FYSGHRSPHI PYKLLHLVWT HARHLAGYEQ QDAHEFLIAA LDVLHRHCKG DDNGKKANNP NHCNCIIDQI FTGGLQSDVT CQVCHGVSTT IDPFWDISLD LPGSSTPFWP LSPGSEGNVV NGESHVSGTT TLTDCLRRFT RPEHLGSSAK IKCSGCHSYQ ESTKQLTMKK LPIVACFHLK RFEHSAKLRR KITTYVSFPL ELDMTPFMAS SKESRMNGQY QQPTDSLNND NKYSLFAVVN HQGTLESGHY TSFIRQHKDQ WFKCDDAIIT KASIKDVLDS EGYLLFYHKQ FLEYE
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Molecular Weight
59.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
USP22, a member of the ubiquitin-specific protease family, plays a crucial role in regulating various cellular processes, including protein degradation, cell cycle progression, and gene expression. Its involvement in the removal of ubiquitin moieties from target proteins highlights its significance in maintaining cellular homeostasis and regulating protein turnover. Recent studies have implicated USP22 in cancer biology, where it acts as an oncogene by enhancing the stability of proteins involved in tumorigenesis and promoting cell survival. Overexpression of USP22 has been associated with poor prognosis in several cancer types, making it a potential therapeutic target. Moreover, its function within the context of the transcriptional regulation of stem cell maintenance and differentiation has garnered interest, as USP22 is linked to the modulation of genes critical for maintaining pluripotency. Given its multifaceted roles, research focused on USP22 recombinant protein aims to elucidate its biochemical properties and interactions, paving the way for the development of targeted therapies. The generation of USP22 recombinant protein provides essential tools for exploring its enzymatic activity, structure-function relationship, and potential as a biomarker in cancer diagnostics. Overall, the investigation of USP22's function at the molecular level is vital for understanding its contribution to disease mechanisms and for harnessing its potential in therapeutic applications.
