Analytical Data
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基因名
CRY1
- Application
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别名
CRY1；PHLL1；Cryptochrome-1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q16526
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表达区间
1-586aa
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氨基酸序列
MGVNAVHWFRKGLRLHDNPALKECIQGADTIRCVYILDPWFAGSSNVGIN RWRFLLQCLEDLDANLRKLNSRLFVIRGQPADVFPRLFKEWNITKLSIEY DSEPFGKERDAAIKKLATEAGVEVIVRISHTLYDLDKIIELNGGQPPLTY KRFQTLISKMEPLEIPVETITSEVIEKCTTPLSDDHDEKYGVPSLEELGF DTDGLSSAVWPGGETEALTRLERHLERKAWVANFERPRMNANSLLASPTG LSPYLRFGCLSCRLFYFKLTDLYKKVKKNSSPPLSLYGQLLWREFFYTAA TNNPRFDKMEGNPICVQIPWDKNPEALAKWAEGRTGFPWIDAIMTQLRQE GWIHHLARHAVACFLTRGDLWISWEEGMKVFEELLLDADWSINAGSWMWL SCSSFFQQFFHCYCPVGFGRRTDPNGDYIRRYLPVLRGFPAKYIYDPWNA PEGIQKVAKCLIGVNYPKPMVNHAEASRLNIERMKQIYQQLSRYRGLGLL ASVPSNPNGNGGFMGYSAENIPGCSSSGSCSQGSGILHYAHGDSQQTHLL KQGRSSMGTGLSGGKRPSQEEDTQSIGPKVQRQSTN
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分子量
90 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CRY1, or Cryptochrome 1, is a pivotal protein involved in the regulation of circadian rhythms and various physiological processes in organisms ranging from plants to mammals. Originally identified as a photoreceptor that mediates the effects of light on biological clocks, CRY1 plays a crucial role in the synchronization of the circadian cycle with environmental light-dark cycles. Research has demonstrated that CRY1 not only influences circadian rhythms but also participates in the regulation of gene expression, cellular signaling pathways, and responses to environmental stress. The investigation of recombinant CRY1 proteins has gained significant attention in the fields of chronobiology and molecular biology, as these proteins are essential for elucidating the mechanistic insights of light perception and circadian regulation. Recombinant CRY1 proteins, produced through techniques such as recombinant DNA technology, allow scientists to study the protein's structure-function relationships and interactions with other molecular partners in vitro. Understanding CRY1's role in these processes is vital for uncovering the underlying mechanisms driving circadian biology, with implications for health, agriculture, and the development of therapeutics targeting circadian-related disorders. Overall, research on CRY1 recombinant proteins continues to provide essential insights into the complexity of circadian biology and its influence on various biological systems.
