Analytical Data
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Gene name
ADAMTS5
- Application
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Alternative Names
ADAMTS5；OPT；Opticin
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9UNA0
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Expression Region
262-930aa
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AA Sequence
SISRARQVE LLLVADASMA RLYGRGLQHY LLTLASIANR LYSHASIENH IRLAVVKVVV LGDKDKSLEV SKNAATTLKN FCKWQHQHNQ LGDDHEEHYD AAILFTREDL CGHHSCDTLG MADVGTICSP ERSCAVIEDD GLHAAFTVAH EIGHLLGLSH DDSKFCEETF GSTEDKRLMS SILTSIDASK PWSKCTSATI TEFLDDGHGN CLLDLPRKQI LGPEELPGQT YDATQQCNLT FGPEYSVCPG MDVCARLWCA VVRQGQMVCL TKKLPAVEGT PCGKGRICLQ GKCVDKTKKK YYSTSSHGNW GSWGSWGQCS RSCGGGVQFA YRHCNNPAPR NNGRYCTGKR AIYRSCSLMP CPPNGKSFRH EQCEAKNGYQ SDAKGVKTFV EWVPKYAGVL PADVCKLTCR AKGTGYYVVF SPKVTDGTEC RLYSNSVCVR GKCVRTGCDG IIGSKLQYDK CGVCGGDNSS CTKIVGTFNK KSKGYTDVVR IPEGATHIKV RQFKAKDQTR FTAYLALKKK NGEYLINGKY MISTSETIID INGTVMNYSG WSHRDDFLHG MGYSATKEIL IVQILATDPT KPLDVRYSFF VPKKSTPKVN SVTSHGSNKV GSHTSQPQWV TGPWLACSRT CDTGWHTRTV QCQDGNRKLA KGCPLSQRPS AFKQCLLKKC
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ADAMTS5 (A Disintegrin And Metalloproteinase with Thrombospondin Motifs 5) is a member of the ADAMTS family, which plays a crucial role in the degradation of extracellular matrix components, particularly aggrecan, a key proteoglycan in cartilage. The study of ADAMTS5 is significant in the context of osteoarthritis and other degenerative joint diseases, where its overactivity can lead to the loss of cartilage integrity and contribute to disease progression. Understanding the structure and function of ADAMTS5 is essential for developing therapeutic strategies aimed at inhibiting its activity to slow down cartilage degradation. Recombinant protein studies allow researchers to analyze the enzymatic properties, substrate specificity, and regulatory mechanisms of ADAMTS5 in detail. By generating recombinant ADAMTS5, scientists can explore its interactions with other matrix components and inhibitors, shed light on its physiological functions, and evaluate potential drug candidates that target its activity. These insights may eventually pave the way for novel treatments to protect cartilage health and manage chronic joint conditions effectively. Overall, the research on recombinant ADAMTS5 is crucial for dissecting its role in cartilage homeostasis and potentially finding ways to mitigate the impact of cartilage-related disorders.
