Analytical Data
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基因名
GJB2
- Application
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别名
GJB2; Gap junction beta-2 protein; Connexin-26; Cx26
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P29033
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表达区间
1-226aa
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氨基酸序列
MDWGTLQTILGGVNKHSTSIGKIWLTVLFIFRIMILVVAAKEVWGDEQADFVCNTLQPGCKNVCYDHYFPISHIRLWALQLIFVSTPALLVAMHVAYRRHEKKRKFIKGEIKSEFKDIEEIKTQKVRIEGSLWWTYTSSIFFRVIFEAAFMYVFYVMYDGFSMQRLVKCNAWPCPNTVDCFVSRPTEKTVFTVFMIAVSGICILLNVTELCYLLIRYCSGKSKKPV
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分子量
50.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GJB2, also known as Connexin 26, is a critical component of gap junctions, playing a vital role in cell-to-cell communication, particularly in the inner ear. Mutations in the GJB2 gene are among the most common genetic causes of hereditary non-syndromic sensorineural hearing loss, affecting millions worldwide. Understanding the structure and function of GJB2 is essential for elucidating the mechanisms behind such hearing impairments. Recombinant GJB2 protein studies enable researchers to investigate the channel properties, interaction with other connexins, and the effects of specific mutations on its function. By producing GJB2 protein through recombinant DNA technology, scientists can study its biophysical properties, examine the impact of pathogenic mutations, and explore potential therapeutic strategies to restore hearing function. Additionally, recombinant GJB2 can serve as a valuable tool for drug screening and the development of gene therapies. Overall, the ongoing research into GJB2 recombinant protein not only enhances our understanding of auditory biology but also holds promise for innovative treatments for hearing loss.
