Analytical Data
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基因名
GCNT2
- Application
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别名
GCNT2; GCNT5; II; NACGT1; N-acetyllactosaminide beta-1.6-N-acetylglucosaminyl-transferase; N-acetylglucosaminyltransferase; EC 2.4.1.150; I-branching enzyme; IGNT
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8N0V5
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表达区间
1-402aa
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氨基酸序列
MNFWRYCFFAFTLLSVVIFVRFYSSQLSPPKSYEKLNSSSERYFRKTACNHALEKMPVFLWENILPSPLRSVPCKDYLTQNHYITSPLSEEEAAFPLAYVMVIHKDFDTFERLFRAIYMPQNVYCVHVDEKAPAEYKESVRQLLSCFQNAFIASKTESVVYAGISRLQADLNCLKDLVASEVPWKYVINTCGQDFPLKTNREIVQHLKGFKGKNITPGVLPPDHAIKRTKYVHQEHTDKGGFFVKNTNILKTSPPHQLTIYFGTAYVALTRDFVDFVLRDQRAIDLLQWSKDTYSPDEHFWVTLNRVSGVPGSMPNASWTGNLRAIKWSDMEDRHGGCHGHYVHGICIYGNGDLKWLVNSPSLFANKFELNTYPLTVECLELRHRERTLNQSETAIQPSWYF
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分子量
72.9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GCNT2, or GlcNAc transferase 2, plays a crucial role in glycosylation, specifically in the biosynthesis of complex N-glycans and O-glycans. It is essential for the development of various tissues and organs, influencing cell signaling, adhesion, and immune responses. Aberrant GCNT2 activity has been linked to several diseases, including cancer, diabetes, and inflammatory disorders. Its function is particularly significant in the context of tumor biology, as altered glycosylation patterns can affect tumor progression and metastasis. The recombinant expression of GCNT2 is vital for studying its enzymatic properties, substrate specificity, and regulatory mechanisms. By producing and characterizing GCNT2 as a recombinant protein, researchers aim to elucidate its role in glycan biosynthesis and its implications in health and disease. This understanding could lead to novel therapeutic strategies targeting glycosylation pathways, offering potential interventions in pathological conditions associated with GCNT2 dysregulation.
