Analytical Data
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Gene name
ACVRL1/ALK1
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简介
ALK-1, also known as ACVRL1, is a type I receptor for TGF-β superfamily with 2 ligands, BMP9 and BMP10. ALK-1 is predominantly expressed in endothelial cells and plays a critical role in regulating developmental and pathological angiogenesis[1][2]. ALK-1 Protein, Cynomolgus (HEK293, His) is produced in HEK293 cells with a C-Terminal His-tag. It consists of 118 amino acids (D22-Q118).
- Application
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Biological Activity
Measured by its ability to inhibit BMP-9-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 3.992 ng/mL in the presence of 2 ng/mL of rhBMP-9, corresponding to a specific activity is 2.505×105 U/mg. Measured by its ability to inhibit BMP-9-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 3.992 ng/mL in the presence of 2 ng/mL of rhBMP-9, corresponding to a specific activity is 2.505×105 U/mg.
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Alternative Names
Serine/threonine-protein kinase receptor R3; SKR3; ALK-1; TSR-I; ACVRL1
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Species
Cynomolgus
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Source
HEK293
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Tag
C-His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
XP_005570958
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Expression Region
D22-Q118
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Protein Length
Partial
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Molecular Weight
21-25 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ACVRL1, also known as activin A receptor type II-like 1, is a member of the transforming growth factor-beta (TGF-β) superfamily and plays a crucial role in various biological processes, including vascular development and endothelial function. Mutations in the ACVRL1 gene are linked to hereditary hemorrhagic telangiectasia type 2 (HHT2), a genetic disorder characterized by abnormal blood vessel formation that leads to frequent bleeding and arteriovenous malformations. Research on ACVRL1 has gained importance due to its implications in vascular biology and disease mechanisms. The recombinant ACVRL1 protein has become an essential tool for studying the signaling pathways mediated by this receptor, elucidating its role in angiogenesis and vascular stability. Understanding the structure and function of ACVRL1 can provide insights into potential therapeutic approaches for conditions associated with dysfunctional blood vessel formation. Additionally, by investigating the interactions of ACVRL1 with other signaling molecules and its downstream effects, researchers aim to develop targeted strategies to treat HHT and related vascular anomalies. Thus, the study of recombinant ACVRL1 not only advances our grasp of fundamental vascular biology but also holds promise for developing innovative treatments for vascular-related disorders.
