Analytical Data
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基因名
HK1
- Application
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别名
Brain form hexokinaseHexokinase type I ;HK I
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种属
Human
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表达系统
E. coli
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标签
N- His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P19367
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表达区间
13-475aa
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分子量
55.9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HK1, or hexokinase 1, is a crucial enzyme in the glycolytic pathway, playing a significant role in glucose metabolism by catalyzing the phosphorylation of glucose to glucose-6-phosphate. This enzyme is primarily expressed in various tissues, including the brain, liver, and skeletal muscle, and is essential for maintaining cellular energy homeostasis. Recent research has highlighted the importance of HK1 in cancer biology, where its aberrant expression is linked to increased tumor growth and survival. In cancer cells, HK1 not only facilitates rapid metabolic adaptation but also inhibits apoptosis, allowing for enhanced proliferation under hypoxic conditions. Additionally, studies have revealed potential regulatory mechanisms, such as post-translational modifications and interactions with mitochondrial proteins, that modulate HK1 activity and localization. Given its pivotal role in both metabolism and cell survival, HK1 has emerged as a promising target for therapeutic intervention in various malignancies. Understanding the structural and functional characteristics of recombinant HK1 could provide insights into its regulatory mechanisms and open new avenues for drug development aimed at manipulating its activity for cancer treatment. Overall, ongoing research into HK1, particularly its recombinant protein forms, continues to shed light on its multifaceted roles in metabolic disorders and cancer, underscoring its potential as a target for innovative therapeutic strategies.
