Analytical Data
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Gene name
Calcitonin R
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简介
Calcitonin R protein, a receptor for calcitonin, utilizes G proteins to activate adenylyl cyclase. Despite being sensitive to cholera toxin, it exhibits limited coupling to G proteins, leading to the inability to effectively activate adenylyl cyclase. Notably, following ligand binding, Calcitonin R does not undergo the conventional process of receptor internalization. Calcitonin R Protein, Human (HEK293, hFc) is the recombinant human-derived Calcitonin R, expressed by HEK293 , with N-hFc labeled tag.
- Application
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Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized Human Calcitonin at 2μg/mL (100μL/well) can bind Biotinylated Human Calcitonin R. The ED50 for this effect is 4.532 μg/mL. Measured by its binding ability in a functional ELISA. Immobilized Human Calcitonin at 2μg/mL (100μL/well) can bind Biotinylated Human Calcitonin R. The ED50 for this effect is 4.532μg/mL.
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Alternative Names
Calcitonin R; Calcitonin receptor; CALCR; CRT; CTR; CTR1
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Species
Human
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Source
HEK293
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Tag
N-hFc
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
P30988
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Expression Region
A25-V154
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Protein Length
Partial
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Molecular Weight
55-75 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Calcitonin is a peptide hormone produced by the parafollicular cells of the thyroid gland, primarily involved in calcium homeostasis. Research on recombinant calcitonin (rCT) has gained significant attention due to its potential therapeutic applications in the treatment of various bone-related disorders, such as osteoporosis and Paget's disease. Traditionally, calcitonin was derived from animal sources, which raised concerns about immunogenicity and supply chain limitations. The advent of recombinant DNA technology has allowed for the production of calcitonin in more controllable and safer environments, addressing these concerns. Additionally, rCT has been studied for its effects on bone metabolism, including its ability to inhibit osteoclast activity, thereby reducing bone resorption and enhancing bone density. Given the global rise in osteoporosis cases and the associated healthcare burden, there is a growing interest in developing effective rCT formulations that can be delivered via various routes, including subcutaneous and intranasal administration. Ongoing research aims to optimize the pharmacokinetic properties of rCT, evaluate its long-term efficacy, and explore potential combinations with other therapeutic agents to maximize its effect on bone health. Overall, the synthesis and research of recombinant calcitonin represent a promising avenue for improving treatment strategies for calcium-related disorders.
