Analytical Data
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Gene name
Frizzled-7
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简介
GIP Protein is a protein involved in the regulation of glucose homeostasis and insulin secretion. It plays a crucial role in modulating pancreatic beta-cell function and glucose metabolism. Dysregulation of GIP Protein has been associated with various diseases, including type 2 diabetes and obesity. Targeting GIP Protein may offer potential therapeutic interventions in these conditions by improving glucose regulation and insulin sensitivity. Frizzled-7 Protein, Human (CHO, hFc) is the recombinant human-derived Frizzled-7 protein, expressed by CHO , with C-hFc labeled tag.
- Application
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Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized Human Frizzled-7, at 0.1μg/mL (100 μL/well) can bind Biotinylated Wnt-5a. The ED50 for this effect is 257.8 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Human Frizzled-7, at 0.1 μg/mL (100 μL/well) can bind Biotinylated Wnt-5a.The ED50 for this effect is 257.8ng/mL.
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Alternative Names
Frizzled Class Receptor 7; FzE3; Frizzled 7, Seven Transmembrane Spanning Receptor; Frizzled Family Receptor 7; Frizzled-7
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Species
Human
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Source
CHO
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Tag
C-hFc
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
O75084
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Expression Region
Q33-L185
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Protein Length
Partial
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Molecular Weight
46-60 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Frizzled-7 (Fz7) is a member of the Frizzled family of proteins, which are integral to the Wnt signaling pathway, a crucial mechanism in various developmental processes and tissue homeostasis. In recent years, research has focused on Fz7 due to its significant role in the regulation of cellular functions such as cell proliferation, differentiation, and polarity. Dysregulation of Wnt signaling, often mediated by Frizzled receptors like Fz7, has been implicated in numerous diseases, including cancers and developmental disorders. Therefore, understanding the structure and function of Fz7 is paramount for elucidating the molecular mechanisms underpinning these pathologies. The study of Fz7 recombinant proteins has gained traction as it allows researchers to investigate the receptor's binding affinities, signaling capabilities, and interactions with Wnt ligands and other intracellular partners in a controlled environment. Additionally, recombinant Fz7 proteins can serve as valuable tools for the development of therapeutic strategies aimed at modulating Wnt signaling. By employing techniques such as protein expression in heterologous systems and subsequent purification, researchers aim to characterize the biochemical properties of Fz7 further. This knowledge can pave the way for potential interventions in conditions associated with aberrant Wnt signaling, highlighting the importance of Frizzled-7 in both basic biology and clinical applications.
