Analytical Data
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Gene name
pncA
- Application
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Alternative Names
(Nicotinamide deamidase)(NAMase)(Pyrazinamidase)(PZAase)
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Species
Escherichia coli
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P21369
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Expression Region
1-213aa
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Molecular Weight
30.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PNCA, or pyrazinamidase/Nicatinamide adenine dinucleotide (NAD) hydrolase, is an essential enzyme in Mycobacterium tuberculosis, responsible for the activation of pyrazinamide, a crucial anti-tuberculosis drug. Understanding the functional dynamics of recombinant PNCa is vital for elucidating the mechanisms of drug resistance and the bacterium's metabolic pathways. Research into PNCa has gained momentum due to the rising incidence of multidrug-resistant tuberculosis, which poses significant challenges to global public health. Studies have shown that mutations in the pncA gene can lead to resistance against pyrazinamide, making it imperative to characterize the enzyme's structure and function. The recombinant expression of PNCa allows researchers to investigate its biochemical properties, substrate specificity, and interaction with inhibitors, ultimately aiming to identify potential therapeutic targets. Additionally, the exploration of PNCa's role in metabolism can shed light on its contribution to the survival and virulence of M. tuberculosis under varying environmental conditions. This research not only enhances our understanding of the pathogen's biology but also contributes to the development of more effective treatment strategies against tuberculosis. As such, the investigation of recombinant PNCa is a crucial step toward overcoming the challenges posed by antibiotic resistance and improving patient outcomes in tuberculosis treatment.
