Analytical Data
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基因名
rpsC
- Application
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别名
rpsC;ECF RNA polymerase sigma factor SigC
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P0A7V3
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表达区间
2-233aa
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氨基酸序列
GQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLTKELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQINIAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGAEIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPEKPAAQPKKQQRKGRK
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分子量
52.9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Ribosomal protein S3 (RpsC) is a crucial component of the ribosomal machinery, playing a vital role in protein synthesis within prokaryotic organisms. As part of the 30S ribosomal subunit, RpsC is involved in the decoding of mRNA and the proper assembly of ribosomes, making it essential for translation accuracy and efficiency. The study of RpsC recombinant proteins has significant implications in understanding ribosome biogenesis, the mechanisms of antibiotic action, and the development of new therapeutic strategies. In particular, RpsC and its interactions with other ribosomal proteins and RNA are critical for the structural integrity and function of ribosomes. Through recombinant DNA technology, researchers have been able to produce RpsC in heterologous systems, allowing for detailed biophysical and biochemical characterization. This has led to insights into the protein’s structural features, conformational dynamics, and binding interactions with ribosomal RNA and other translational factors. Moreover, understanding the modifications and variations of RpsC among different bacterial species can shed light on evolutionary mechanisms and potential vulnerabilities that could be targeted in drug development. As antibiotic resistance continues to pose significant challenges in medicine, elucidating the role of RpsC in ribosomal function offers pathways to discover novel inhibitors that can combat resistant strains, thereby highlighting the importance of ongoing research into this pivotal ribosomal protein.












