Analytical Data
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基因名
rplF
- Application
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别名
rplF;Large ribosomal subunit Protein uL6
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P0AG55
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表达区间
2-175aa
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氨基酸序列
SRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVKHADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNVINLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGKGVRYADEVVRTKEAK
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分子量
45.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RplF, a critical protein component of the ribosomal subunit, plays an essential role in the translation process of protein synthesis in bacteria. As a part of the 50S ribosomal subunit, RplF is involved in the assembly and function of the ribosome, facilitating the interaction between ribosomal RNA and various proteins. Research on RplF has garnered significant attention due to its potential implications in antibiotic resistance, as many antibiotics target bacterial ribosomes to inhibit protein synthesis. Understanding the structure and function of RplF can provide insights into the mechanisms of ribosome assembly and the effects of ribosomal inhibitors. Furthermore, studies exploring the recombinant expression of RplF allow for the production of sufficient quantities of the protein for further biochemical and structural analyses. Through these investigations, researchers aim to elucidate the protein's role in ribosome biogenesis and its interactions with antibiotics, ultimately paving the way for developing novel therapeutic strategies against bacterial infections. This research is especially crucial in the context of rising antibiotic resistance, where enhancing our understanding of ribosomal components like RplF may lead to innovative approaches in combating resistant bacterial strains. Thus, the exploration of RplF not only contributes to fundamental molecular biology but also holds significant promise for applications in drug development and the fight against infectious diseases.












