Analytical Data
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Gene name
NDK1
- Application
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Alternative Names
NDK1；NDPK1；Nucleoside diphosphate kinase 1
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Species
Mouse
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P39207
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Expression Region
1-149aa
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AA Sequence
MEQTFIMIKPDGVQRGLIGEVICRFEKKGFTLKGLKLISVERSFAEKHYEDLSSKSFFSGLVDYIVSGPVVAMIWEGKNVVLTGRKIIGATNPAASEPGTIRGDFAIDIGRNVIHGSDSVESARKEIALWFPDGPVNWQSSVHPWVYET
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Molecular Weight
20.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NDK1 (Nucleoside Diphosphate Kinase 1) is a critical enzyme involved in nucleotide metabolism, playing a key role in the regulation of intracellular nucleotide pools and energy balance. It catalyzes the conversion of nucleoside diphosphates to triphosphates, facilitating DNA and RNA synthesis, which is essential for cell proliferation and survival. Research has highlighted the significance of NDK1 in various biological processes, including cell signaling, apoptosis, and stress response. Abnormal expression or activity of NDK1 has been implicated in several diseases, including cancer, where it may contribute to tumorigenesis by enhancing cell proliferation and survival under hostile conditions. Therefore, the recombinant expression of NDK1 protein is of considerable interest for both fundamental studies and potential therapeutic applications. By producing NDK1 in a controlled laboratory environment, researchers can investigate its biochemical properties, regulatory mechanisms, and interactions with other cellular components. This foundational understanding can lead to the development of targeted therapies that modulate NDK1 activity in disease contexts, indicating its potential as a biomarker or therapeutic target in oncology and other disorders linked to nucleotide metabolism. The development of recombinant NDK1 also provides a platform for high-throughput screening of small molecules that could inhibit or enhance its enzymatic activity, further advancing our understanding of its role in cellular physiology and pathology. Overall, the study of recombinant NDK1 is not only vital for elucidating its biological functions but also holds promise for novel therapeutic strategies aimed at manipulating nucleotide metabolism in disease.
