Analytical Data
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Gene name
EXOSC5
- Application
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Alternative Names
EXOSC5；CML28；RRP46;Exosome complex component RRP46
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NQT4
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Expression Region
1-235aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MMEEETHTDA KIRAENGTGS SPRGPGCSLR HFACEQNLLS RPDGSASFLQ GDTSVLAGVY GPAEVKVSKE IFNKATLEVI LRPKIGLPGV AEKSRERLIR NTCEAVVLGT LHPRTSITVV LQVVSDAGSL LACCLNAACM ALVDAGVPMR ALFCGVACAL DSDGTLVLDP TSKQEKEARA VLTFALDSVE RKLLMSSTKG LYSDTELQQC LAAAQAASQH VFRFYRESLQ RRYSKS
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Molecular Weight
28 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
EXOSC5, part of the EXOSC (exosome complex) family, is a crucial component involved in RNA processing and degradation, playing a significant role in the regulation of various cellular processes. It is predominantly recognized for its function in the exosome complex, a multi-protein complex that degrades RNA molecules in a 3' to 5' direction, thereby influencing gene expression and RNA turnover. Recent studies have highlighted the importance of EXOSC5 in maintaining cellular homeostasis and its involvement in pathophysiological conditions. Notably, mutations in the EXOSC5 gene have been associated with specific disorders, including neurodegenerative diseases and developmental syndromes such as pontocerebellar hypoplasia. Understanding the biochemical properties and functionalities of EXOSC5 through recombinant protein studies is essential for elucidating its role in RNA metabolism and its contributions to disease mechanisms. The production of EXOSC5 as a recombinant protein allows for in-depth functional analyses and characterization of its enzymatic activity, protein interactions, and structural features. This research not only enhances our fundamental knowledge of RNA processing but also paves the way for potential therapeutic strategies targeting EXOSC5-related pathways in various disorders, highlighting the protein's significance in both basic and applied biomedical research.
