Analytical Data
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Gene name
E189K
- Application
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Alternative Names
E189K；
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P09385
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Expression Region
23-319aa
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AA Sequence
REFTIDFSTQQSYVSSLNSIRTEISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAGFVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSSYLALMEFSGNTMTRDASRAVLRFVTVTAKALRFRQIQREFRQALSETAPVYTMTPGDVDLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNEESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK
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Molecular Weight
40.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The E189K recombinant protein has garnered significant interest within the scientific community due to its potential implications in understanding various biochemical processes and disease mechanisms. This protein variant is derived from a specific mutation found in certain biological systems, which has been linked to altered protein function and cellular pathways. The study of E189K aims to elucidate the structural and functional consequences of this mutation, thereby providing insights into its role in pathological conditions, such as specific cancers or genetic disorders. By employing techniques such as site-directed mutagenesis, molecular modeling, and functional assays, researchers are exploring the protein’s stability, interaction with other biomolecules, and overall impact on cellular activity. Furthermore, the E189K recombinant protein serves as a model for drug discovery efforts aimed at targeting mutated proteins, presenting opportunities for therapeutic interventions. Understanding the properties and behaviors of this protein variant is not only crucial for basic biology but also for the development of personalized medicine approaches that could enhance treatment strategies for affected individuals. Through these investigations, the E189K protein may reveal critical insights into the mechanisms of disease and pave the way for innovative therapeutic solutions.
