Analytical Data
-
Gene name
LCMT1
- Application
-
Alternative Names
LCMT1;LCMT;Leucine carboxyl methyltransferase 1
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q9UIC8
-
Expression Region
1-334aa
-
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMATRQR ESSITSCCST SSCDADDEGV RGTCEDASLC KRFAVSIGYW HDPYIQHFVR LSKERKAPEI NRGYFARVHG VSQLIKAFLR KTECHCQIVN LGAGMDTTFW RLKDEDLLPS KYFEVDFPMI VTRKLHSIKC KPPLSSPILE LHSEDTLQMD GHILDSKRYA VIGADLRDLS ELEEKLKKCN MNTQLPTLLI AECVLVYMTP EQSANLLKWA ANSFERAMFI NYEQVNMGDR FGQIMIENLR RRQCDLAGVE TCKSLESQKE RLLSNGWETA SAVDMMELYN RLPRAEVSRI ESLEFLDEME LLEQLMRHYC LCWATKGGNE LGLKEITY
-
Molecular Weight
41 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
LCMT1 (Leucine Carboxyl Methyltransferase 1) is an important enzyme involved in the post-translational modification of proteins through the methylation of the carboxyl terminus of substrate proteins, particularly during protein synthesis and maturation. The enzyme plays a crucial role in regulating various cellular processes, including signal transduction, cellular growth, and differentiation, thereby influencing cellular responses to stress and external stimuli. Research into LCMT1 has gained traction due to its involvement in several diseases, including cancer and neurodegenerative disorders, highlighting its potential as a therapeutic target. The reconstitution of LCMT1 as a recombinant protein facilitates detailed studies of its biochemical properties, substrate specificity, and functional mechanisms. This approach also allows for the exploration of its interactions with other cellular components and elucidation of its role in cellular signaling pathways. Understanding the structure and function of LCMT1 at a molecular level can aid in the development of small molecule inhibitors or modulators that could potentially lead to novel treatment strategies. Overall, the investigation of LCMT1 recombinant protein is pivotal for advancing our knowledge of its biological significance and therapeutic potential in human health and disease.
