Cat: PA2000-401DB

Recombinant Human NAD Protein,His

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Analytical Data

  • Gene name

    NAD

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    NAD;SIR2L;SIR2L2;NAD-dependent Protein deacetylase sirtuin-2

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q8IXJ6

  • Expression Region

    2-389aa

  • AA Sequence

    AEPDPSHPL ETQAGKVQEA QDSDSDSEGG AAGGEADMDF LRNLFSQTLS LGSQKERLLD ELTLEGVARY MQSERCRRVI CLVGAGISTS AGIPDFRSPS TGLYDNLEKY HLPYPEAIFE ISYFKKHPEP FFALAKELYP GQFKPTICHY FMRLLKDKGL LLRCYTQNID TLERIAGLEQ EDLVEAHGTF YTSHCVSASC RHEYPLSWMK EKIFSEVTPK CEDCQSLVKP DIVFFGESLP ARFFSCMQSD FLKVDLLLVM GTSLQVQPFA SLISKAPLST PRLLINKEKA GQSDPFLGMI MGLGGGMDFD SKKAYRDVAW LGECDQGCLA LAELLGWKKE LEDLVRREHA SIDAQSGAGV PNPSTSASPK KSPPPAKDEA RTTEREKPQ

  • Molecular Weight

    43.1 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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Protein Description

NAD (Nicotinamide Adenine Dinucleotide) is a crucial coenzyme found in all living cells, playing a key role in various metabolic processes, including cellular respiration and energy production. Recent studies have emphasized the significance of NAD in maintaining cellular homeostasis and its involvement in redox reactions. The research on NAD and its reconstituted proteins has gained momentum due to the emerging evidence that NAD is not only pivotal in energy metabolism but also in regulating cellular aging and stress resistance. NAD-dependent enzymes, such as sirtuins and poly(ADP-ribose) polymerases (PARPs), have become focal points of study, linking NAD levels to aging, neurodegenerative diseases, and metabolic disorders. Understanding the reconstitution of NAD-dependent proteins aids in unraveling their biological functions and can offer potential therapeutic strategies for age-related conditions and metabolic diseases. Over the past few years, advances in protein engineering and synthetic biology have enabled researchers to create NAD-reconstituted proteins with tailored functionalities, paving the way for innovative applications in biotechnology and medicine. This growing body of research underscores the importance of NAD in both fundamental biology and therapeutic innovation, highlighting a promising avenue for future scientific exploration.

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