Analytical Data
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基因名
AMPK Alpha 1
- Application
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别名
AMPK Alpha 1；5'-AMP-activated Protein kinase subunit gamma-1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q13131
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表达区间
1-559aa
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氨基酸序列
MRRLSSWRKM ATAEKQKHDG RVKIGHYILG DTLGVGTFGK VKVGKHELTG HKVAVKILNR QKIRSLDVVG KIRREIQNLK LFRHPHIIKL YQVISTPSDI FMVMEYVSGG ELFDYICKNG RLDEKESRRL FQQILSGVDY CHRHMVVHRD LKPENVLLDA HMNAKIADFG LSNMMSDGEF LRTSCGSPNY AAPEVISGRL YAGPEVDIWS SGVILYALLC GTLPFDDDHV PTLFKKICDG IFYTPQYLNP SVISLLKHML QVDPMKRATI KDIREHEWFK QDLPKYLFPE DPSYSSTMID DEALKEVCEK FECSEEEVLS CLYNRNHQDP LAVAYHLIID NRRIMNEAKD FYLATSPPDS FLDDHHLTRP HPERVPFLVA ETPRARHTLD ELNPQKSKHQ GVRKAKWHLG IRSQSRPNDI MAEVCRAIKQ LDYEWKVVNP YYLRVRRKNP VTSTYSKMSL QLYQVDSRTY LLDFRSIDDE ITEAKSGTAT PQRSGSVSNY RSCQRSDSDA EAQGKSSEVS LTSSVTSLDS SPVDLTPRPG SHTIEFFEMC ANLIKILAQ
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分子量
64 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
AMP-activated protein kinase (AMPK) is a crucial energy sensor in cells, playing a vital role in maintaining cellular energy homeostasis. The AMPK heterotrimeric complex consists of a catalytic alpha (α) subunit and regulatory beta (β) and gamma (γ) subunits. Among the two isoforms of the alpha subunit, AMPK alpha 1 (AMPKα1) has garnered significant attention due to its involvement in various metabolic processes, including glucose uptake, fatty acid oxidation, and the regulation of mitochondrial biogenesis. Research has shown that AMPKα1 is activated in response to increased levels of AMP, facilitating the switch from anabolic to catabolic pathways, particularly during stress conditions such as exercise or nutrient deprivation. Furthermore, AMPKα1 has been associated with the pathophysiology of several diseases, including obesity, diabetes, and cancer, highlighting its potential as a therapeutic target. The exploration of AMPKα1 recombinant protein is crucial for elucidating its role in cellular metabolism and signaling pathways. By studying the structure, function, and regulatory mechanisms of AMPKα1, researchers aim to develop new strategies for modulating its activity, thereby influencing metabolic health and addressing metabolic disorders. The production of AMPKα1 recombinant protein allows for detailed biochemical and biophysical analyses, paving the way for potential therapeutic interventions that leverage the AMPK pathway to restore metabolic balance and improve overall health outcomes.
