Analytical Data
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基因名
NBC1
- Application
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别名
SLC4A4;NBC;NBC1;NBCE1;Electrogenic sodium bicarbonate cotransporter 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9Y6R1
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表达区间
1-580aa
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氨基酸序列
MEDEAVLDRGASFLKHVCDEEEVEGHHTIYIGVHVPKSYRRRRRHKRKTGHKEKKEKERISENYSDKSDIENADESSSSILKPLISPAAERIRFILGEEDDSPAPPQLFTELDELLAVDGQEMEWKETARWIKFEEKVEQGGERWSKPHVATLSLHSLFELRTCMEKGSIMLDREASSLPQLVEMIVDHQIETGLLKPELKDKVTYTLLRKHRHQTKKSNLRSLADIGKTVSSASRMFTNPDNGSPAMTHRNLTSSSLNDISDKPEKDQLKNKFMKKLPRDAEASNVLVGEVDFLDTPFIAFVRLQQAVMLGALTEVPVPTRFLFILLGPKGKAKSYHEIGRAIATLMSDEVFHDIAYKAKDRHDLIAGIDEFLDEVIVLPPGEWDPAIRIEPPKSLPSSDKRKNMYSGGENVQMNGDTPHDGGHGGGGHGDCEELQRTGRFCGGLIKDIKRKAPFFASDFYDALNIQALSAILFIYLATVTNAITFGGLLGDATDNMQGVLESFLGTAVSGAIFCLFAGQPLTILSSTGPVLVFERLLFNFSKDNNFDYLEFRLWIGLWSAFLCLILVATDASFLVQYF
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NBC1 (SLC4A4) is a member of the solute carrier family and functions as a sodium-bicarbonate cotransporter, playing a crucial role in maintaining acid-base balance in various tissues, particularly in the kidneys and the central nervous system. The study of NBC1 recombinant protein has gained significant interest due to its involvement in physiological processes linked to pH regulation and bicarbonate transport, which are vital in conditions such as metabolic acidosis and certain neurological disorders. Research indicates that alterations in NBC1 expression or function can lead to disorders like proximal renal tubular acidosis and may influence neurodegenerative conditions. Understanding the structural and functional properties of NBC1 through recombinant techniques provides insights into its mechanism of action and potential therapeutic targets. Advancements in this area could pave the way for novel treatments for acid-base balance disorders and offer a deeper understanding of NBC1's role in cellular physiology and pathophysiology. The generation of NBC1 recombinant protein allows for detailed biochemical assays, characterization, and potential drug screening, thus making it a focal point of research aimed at addressing unmet clinical needs related to bicarbonate transport and associated diseases.
