Analytical Data
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Gene name
NCF1
- Application
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Alternative Names
NCF1;NOXO2;SH3PXD1A;Neutrophil cytosol factor 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P14598
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Expression Region
1-390aa
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AA Sequence
MGDTFIRHIALLGFEKRFVPSQHYVYMFLVKWQDLSEKVVYRRFTEIYEF HKTLKEMFPIEAGAINPENRIIPHLPAPKWFDGQRAAENRQGTLTEYCST LMSLPTKISRCPHLLDFFKVRPDDLKLPTDNQTKKPETYLMPKDGKSTAT DITGPIILQTYRAIANYEKTSGSEMALSTGDVVEVVEKSESGWWFCQMKA KRGWIPASFLEPLDSPDETEDPEPNYAGEPYVAIKAYTAVEGDEVSLLEG EAVEVIHKLLDGWWVIRKDDVTGYFPSMYLQKSGQDVSQAQRQIKRGAPP RRSSIRNAHSIHQRSRKRLSQDAYRRNSVRFLQQRRRQARPGPQSPGSPL EEERQTQRSKPQPAVPPRPSADLILNRCSESTKRKLASAVVEHHHHHH
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Molecular Weight
46 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NCF1, or Neutrophil Cytosolic Factor 1, is a crucial component of the NADPH oxidase complex, which plays a significant role in the immune response by producing reactive oxygen species (ROS). The research on NCF1 recombinant protein has gained prominence due to its essential function in phagocyte activation and microbial elimination. Mutations or deficiencies in the NCF1 gene are linked to Chronic Granulomatous Disease (CGD), a genetically inherited condition that predisposes individuals to recurrent bacterial and fungal infections. Understanding the structure and function of NCF1 is vital for developing targeted therapies for CGD and related disorders. The recombinant production of NCF1 allows researchers to investigate its biochemical properties, interaction with other proteins in the oxidase complex, and its regulatory mechanisms. Additionally, recombinant NCF1 can serve as a valuable tool for in vitro studies to explore the pathways involved in oxidative burst and immune response. This research not only enhances our comprehension of innate immunity but also opens avenues for therapeutic interventions in CGD and potentially other immune-related conditions.
