Analytical Data
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Gene name
NSDHL
- Application
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Alternative Names
NSDHL;H105E3;Sterol-4-alpha-carboxylate 3-dehydrogenase. decarboxylating
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q15738
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Expression Region
1-373aa
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AA Sequence
MEPAVSEPMRDQVARTHLTEDTPKVNADIEKVNQNQAKRCTVIGGSGFLGQHMVEQLLARGYAVNVFDIQQGFDNPQVRFFLGDLCSRQDLYPALKGVNTVFHCASPPPSSNNKELFYRVNYIGTKNVIETCKEAGVQKLILTSSASVIFEGVDIKNGTEDLPYAMKPIDYYTETKILQERAVLGANDPEKNFLTTAIRPHGIFGPRDPQLVPILIEAARNGKMKFVIGNGKNLVDFTFVENVVHGHILAAEQLSRDSTLGGKAFHITNDEPIPFWTFLSRILTGLNYEAPKYHIPYWVAYYLALLLSLLVMVISPVIQLQPTFTPMRVALAGTFHYYSCERAKKAMGYQPLVTMDDAMERTVQSFRHLRRVK
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NSDHL (NAD(P)H-dependent steroid dehydrogenase-like) is an enzyme that plays a critical role in the late stages of cholesterol biosynthesis and the metabolism of sterols. Mutations in the NSDHL gene are associated with X-linked dominant chondrodysplasia punctata (XLDCP), a disorder characterized by skeletal abnormalities and distinctive skin lesions. Understanding the structure and function of NSDHL is essential for elucidating the biochemical pathways involved in cholesterol metabolism and related diseases. Research on NSDHL recombinantly expressed proteins has become increasingly important in the field of molecular biology and biochemistry. By producing and characterizing NSDHL in vitro, scientists can investigate its enzymatic activities, substrate specificity, and interactions with other proteins involved in sterol biosynthesis. This research not only sheds light on the pathological mechanisms underlying XLDCP but also holds potential implications for therapeutic strategies targeting cholesterol-related disorders. Advances in recombinant protein technology have facilitated the expression of NSDHL in various host systems, leading to improved purification methods and functional assays. These developments contribute to a deeper understanding of NSDHL's role in cellular metabolism and may pave the way for novel treatments for patients with genetic mutations affecting this crucial enzyme. Ultimately, studying NSDHL and its recombined forms is vital for expanding our knowledge of lipid metabolism and developing interventions for related diseases.
