Analytical Data
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基因名
PKLR
- Application
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别名
PKLR;PK1;PKL;Pyruvate kinase PKLR
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P30613
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表达区间
2-574aa
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氨基酸序列
SIQENISSLQLRSWVSKSQRDLAKSILIGAPGGPAGYLRRASVAQLTQEL GTAFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSV ERLKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESFAGSPLSYRPV AIALDTKGPEIRTGILQGGPESEVELVKGSQVLVTVDPAFRTRGNANTVW VDYPNIVRVVPVGGRIYIDDGLISLVVQKIGPEGLVTQVENGGVLGSRKG VNLPGAQVDLPGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRAAL GPEGHGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKVFL AQKMMIGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVANAVLDGADC IMLSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRDP TEVTAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRSAQ AARQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDL VIVVTGWRPGSGYTNIMRVLSIS
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分子量
63 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of PKLR (Pyruvate Kinase Liver and Red Cell) recombinant protein is rooted in the critical role of pyruvate kinase in cellular metabolism, specifically in glycolysis, where it facilitates the conversion of phosphoenolpyruvate to pyruvate while generating ATP. PKLR exists as two isoforms—the liver and the red blood cell type—each playing a significant role in energy metabolism in different tissues. Mutations and dysregulation of PKLR have been implicated in various metabolic disorders, including hereditary pyruvate kinase deficiency, leading to hemolytic anemia and related complications. Understanding the structure and function of PKLR is essential for elucidating its pathophysiological roles and developing potential therapeutic strategies. Advances in recombinant protein technology have enabled the production of functional PKLR for in-depth studies, allowing researchers to explore its enzymatic properties, regulation, and interactions with other metabolic pathways. Additionally, the recombinant PKLR protein serves as an important tool in drug discovery and the development of enzymatic assays, further amplifying its significance in biomedical research. As the understanding of PKLR continues to evolve, it offers potential insights into novel therapeutic approaches for metabolic diseases linked to pyruvate kinase dysfunction.
