Analytical Data
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基因名
MLCK
- Application
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别名
zgc:92755; atrial isoform; cmlc2; MGC92755; MLC-2a; MLC2a; MLCK; cardiac; MLRA_HUMAN; MYL2A; MYL7; Mylc2a; Myosin light chain 2a; Myosin light chain 7 regulatory; Myosin light polypeptide 7 regulatory; Myosin regulatory light chain 2; Myosin regulatory light chain 2 atrial isoform; Myosin regulatory light chain 7
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q01449
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表达区间
1-175 aa
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氨基酸序列
MASRKAGTRGKVAATKQAQRGSSNVFSMFEQAQIQEFKEAFSCIDQNRDGIICKADLRETYSQLGKVSVPEEELDAMLQEGKGPINFTVFLTLFGEKLNGTDPEEAILSAFRMFDPSGKGVVNKDEFKQLLLTQADKFSPAEVEQMFALTPMDLAGNIDYKSLCYIITHGDEKEE
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分子量
46.4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Myosin light chain kinase (MLCK) is a crucial enzyme that plays a significant role in cellular processes, particularly in muscle contraction and various signaling pathways. It phosphorylates the myosin light chain, which is essential for muscle contraction in both smooth and striated muscles, thereby regulating smooth muscle contraction and influencing cellular functions such as motility and contraction in non-muscle cells. The understanding of MLCK’s structure and function has important implications in various physiological and pathological conditions, including cardiovascular diseases, asthma, and cancer. Research on MLCK recombinant proteins has gained momentum as scientists strive to elucidate the intricate details of its mechanism of action, post-translational modifications, and interactions with various cellular partners. By producing and purifying MLCK as a recombinant protein, researchers aim to perform in-depth biochemical studies that can shed light on its regulatory mechanisms and potential therapeutic targets. Moreover, studies on MLCK variants and their effects on muscle function can provide insights into the development of muscle-related diseases and pave the way for novel interventions. Overall, the investigation of MLCK through recombinant protein technology is a promising avenue for developing a deeper understanding of its biological roles and therapeutic potential in various diseases.
