Analytical Data
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基因名
DARS2
- Application
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别名
DARS2；Aspartate--tRNA ligase. mitochondrial
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q6PI48
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表达区间
48-645aa
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氨基酸序列
VVR TNTCGELRSS HLGQEVTLCG WIQYRRQNTF LVLRDFDGLV QVIIPQDESA ASVKKILCEA PVESVVQVSG TVISRPAGQE NPKMPTGEIE IKVKTAELLN ACKKLPFEIK NFVKKTEALR LQYRYLDLRS FQMQYNLRLR SQMVMKMREY LCNLHGFVDI ETPTLFKRTP GGAKEFLVPS REPGKFYSLP QSPQQFKQLL MVGGLDRYFQ VARCYRDEGS RPDRQPEFTQ IDIEMSFVDQ TGIQSLIEGL LQYSWPNDKD PVVVPFPTMT FAEVLATYGT DKPDTRFGMK IIDISDVFRN TEIGFLQDAL SKPHGTVKAI CIPEGAKYLK RKDIESIRNF AADHFNQEIL PVFLNANRNW NSPVANFIME SQRLELIRLM ETQEEDVVLL TAGEHNKACS LLGKLRLECA DLLETRGVVL RDPTLFSFLW VVDFPLFLPK EENPRELESA HHPFTAPHPS DIHLLYTEPK KARSQHYDLV LNGNEIGGGS IRIHNAELQR YILATLLKED VKMLSHLLQA LDYGAPPHGG IALGLDRLIC LVTGSPSIRD VIAFPKSFRG HDLMSNTPDS VPPEELKPYH IRVSKPTDSK AERAH
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分子量
73.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DARS2, or mitochondrial aspartyl-tRNA synthetase 2, is a crucial enzyme involved in the mitochondrial protein synthesis pathway. It plays a significant role in charging mitochondrial aspartyl-tRNA with aspartic acid, a process essential for the proper translation of mitochondrial genes. Mutations or dysfunctions in DARS2 can lead to a variety of mitochondrial disorders, including neurodegenerative diseases, characterized by symptoms such as muscle weakness, developmental delays, and neurological impairments. Research on DARS2 recombinants has gained momentum as scientists aim to elucidate its structure-function relationship and the molecular mechanisms underlying its interactions with tRNA and amino acids. Understanding DARS2's role in mitochondrial biology is vital, not only for developing potential therapeutic strategies for related mitochondrial diseases but also for gaining insights into the evolution and adaptation of mitochondria as semi-autonomous organelles. With advances in protein engineering and structural biology, studies on DARS2 recombination are paving the way for potential interventions in mitochondrial dysfunctions, making it a focal point of interest within the fields of molecular biology and genetic medicine.
