Analytical Data
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Gene name
NARS2
- Application
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Alternative Names
NARS2；Asparaginyl-tRNA synthetase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96I59
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Expression Region
15-477aa
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AA Sequence
SSAPFP KHKPSAKLSV RDALGAQNAS GERIKIQGWI RSVRSQKEVL FLHVNDGSSL ESLQVVADSG LDSRELNFGS SVEVQGQLIK SPSKRQNVEL KAEKIKVIGN CDAKDFPIKY KERHPLEYLR QYPHFRCRTN VLGSILRIRS EATAAIHSFF KDSGFVHIHT PIITSNDSEG AGELFQLEPS GKLKVPEENF FNVPAFLTVS GQLHLEVMSG AFTQVFTFGP TFRAENSQSR RHLAEFYMIE AEISFVDSLQ DLMQVIEELF KATTMMVLSK CPEDVELCHK FIAPGQKDRL EHMLKNNFLI ISYTEAVEIL KQASQNFTFT PEWGADLRTE HEKYLVKHCG NIPVFVINYP LTLKPFYMRD NEDGPQHTVA AVDLLVPGVG ELFGGGLREE RYHFLEERLA RSGLTEVYQW YLDLRRFGSV PHGGFGMGFE RYLQCILGVD NIKDVIPFPR FPHSCLL
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Molecular Weight
54 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NARS2 (asparagine-tRNA ligase 2) is a crucial enzyme involved in mitochondrial protein translation, specifically facilitating the attachment of asparagine to its corresponding tRNA. Recent studies have highlighted its significance in mitochondrial function and cellular metabolism. Disruptions or mutations in NARS2 have been linked to diverse mitochondrial disorders, which can manifest as neurodevelopmental issues and various tissue dysfunctions. Given the essential role of mitochondrial translation in cellular energetics and signaling, the investigation of NARS2 has gained momentum in biomedical research. Understanding the structure and function of NARS2, particularly through the study of its recombinant protein, can provide insights into its catalytic mechanisms and interactions with other mitochondrial components. Additionally, elucidating the functional consequences of NARS2 anomalies may pave the way for potential therapeutic strategies targeting mitochondrial diseases. The generation of recombinant NARS2 protein presents an opportunity to explore its biochemical properties, binding affinities, and regulatory pathways, contributing to a broader understanding of mitochondrial biosynthesis and the development of novel treatments for related disorders.
