Analytical Data
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Gene name
ARHGEF16
- Application
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Alternative Names
ARHGEF 16; ARHGEF16; ARHGG_HUMAN; Ephexin 4; Ephexin-4; EPHEXIN4
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q5VV41
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Expression Region
2-709aa
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AA Sequence
AQRHSDSSL EEKLLGHRFH SELRLDAGGN PASGLPMVRG SPRVRDDAAF QPQVPAPPQP RPPGHEEPWP IVLSTESPAA LKLGTQQLIP KSLAVASKAK TPARHQSFGA AVLSREAARR DPKLLPAPSF SLDDMDVDKD PGGMLRRNLR NQSYRAAMKG LGKPGGQGDA IQLSPKLQAL AEEPSQPHTR SPAKNKKTLG RKRGHKGSFK DDPQLYQEIQ ERGLNTSQES DDDILDESSS PEGTQKVDAT IVVKSYRPAQ VTWSQLPEVV ELGILDQLST EERKRQEAMF EILTSEFSYQ HSLSILVEEF LQSKELRATV TQMEHHHLFS NILDVLGASQ RFFEDLEQRH KAQVLVEDIS DILEEHAEKH FHPYIAYCSN EVYQQRTLQK LISSNAAFRE ALREIERRPA CGGLPMLSFL ILPMQRVTRL PLLMDTLCLK TQGHSERYKA ASRALKAISK LVRQCNEGAH RMERMEQMYT LHTQLDFSKV KSLPLISASR WLLKRGELFL VEETGLFRKI ASRPTCYLFL FNDVLVVTKK KSEESYMVQD YAQMNHIQVE KIEPSELPLP GGGNRSSSVP HPFQVTLLRN SEGRQEQLLL SSDSASDRAR WIVALTHSER QWQGLSSKGD LPQVEITKAF FAKQADEVTL QQADVVLVLQ QEDGWLYGER LRDGETGWFP EDFARFITSR VAVEGNVRRM ERLRVETDV
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Molecular Weight
80 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ARHGEF16, a member of the Rho guanine nucleotide exchange factor (GEF) family, plays a pivotal role in regulating various cellular processes, including cytoskeletal dynamics, cell adhesion, and migration. Recent studies have highlighted its involvement in signaling pathways that control cell proliferation and differentiation, suggesting a crucial function in developmental biology and immune responses. Dysregulation of ARHGEF16 has been linked to several pathologies, including cancer, where it may contribute to tumorigenesis and metastasis by influencing the behavior of cancer cells. Research into the structural and functional properties of ARHGEF16, particularly through the generation of recombinant protein, is essential for understanding its mechanisms of action. By producing this protein in a controlled environment, researchers can investigate its interactions with small GTPases and other molecular partners, elucidating its role in Rho signaling networks. Furthermore, recombinant ARHGEF16 can serve as a valuable tool in drug discovery and development, allowing for the exploration of targeted therapies aimed at modulating its activity in disease contexts. As the field of molecular biology continues to unravel the complexities of signal transduction, ARHGEF16 remains a compelling subject for research, potentially offering insights that could lead to innovative therapeutic strategies.
