Analytical Data
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Gene name
PHLDA3
- Application
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Alternative Names
PHLA3_HUMAN; PHLDA 3; phlda3; Pleckstrin homology like domain family A member 2; Pleckstrin homology like domain family A member 3; Pleckstrin homology-like domain family A member 3; TDAG51/Ipl homolog 1; TDAG51/Ipl homologue 1; TIH 1; TIH1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9Y5J5
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Expression Region
1-127 aa
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AA Sequence
MTAAATATVL KEGVLEKRSG GLLQLWKRKR CVLTERGLQL FEAKGTGGRP KELSFARIKA VECVESTGRH IYFTLVTEGG GEIDFRCPLE DPGWNAQITL GLVKFKNQQA IQTVRARQSL GTGTLVS
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Molecular Weight
13.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PHLDA3 (Pleckstrin Homology-Like Domain Family A Member 3) is a protein that has garnered attention in recent years due to its potential role in various physiological and pathological processes, including cancer. As a member of the pleckstrin homology domain-containing proteins, PHLDA3 is implicated in cellular signaling pathways, particularly those related to cell growth and apoptosis. It is known to exhibit tumor suppressor properties, and its expression is often regulated by growth factors, indicating a critical role in cellular responses to environmental stimuli. Research has shown that PHLDA3 can influence the survival of cells under stress conditions, and its dysregulation may contribute to tumorigenesis. Additionally, studies have reported its involvement in hematopoiesis and the modulation of immune responses. Given these diverse functions, the recombinant expression of PHLDA3 is imperative for elucidating its biological roles and mechanisms of action. Production of recombinant PHLDA3 facilitates detailed investigations into its structural properties, binding interactions, and functional assays, which can ultimately enhance our understanding of its therapeutic potential. Therefore, ongoing research focuses not only on the biochemical characterization of this protein but also on its potential as a biomarker for cancer diagnosis and prognostication, further establishing the significance of PHLDA3 in both basic science and clinical applications.
