Analytical Data
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Gene name
Smad1
- Application
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Alternative Names
Smad1；BSP1；MADH1；MADR1；Mothers against decapentaplegic homolog 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q15797
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Expression Region
1-465aa
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AA Sequence
MNVTSLFSFT SPAVKRLLGW KQGDEEEKWA EKAVDALVKK LKKKKGAMEE LEKALSCPGQ PSNCVTIPRS LDGRLQVSHR KGLPHVIYCR VWRWPDLQSH HELKPLECCE FPFGSKQKEV CINPYHYKRV ESPVLPPVLV PRHSEYNPQH SLLAQFRNLG QNEPHMPLNA TFPDSFQQPN SHPFPHSPNS SYPNSPGSSS STYPHSPTSS DPGSPFQMPA DTPPPAYLPP EDPMTQDGSQ PMDTNMMAPP LPSEINRGDV QAVAYEEPKH WCSIVYYELN NRVGEAFHAS STSVLVDGFT DPSNNKNRFC LGLLSNVNRN STIENTRRHI GKGVHLYYVG GEVYAECLSD SSIFVQSRNC NYHHGFHPTT VCKIPSGCSL KIFNNQEFAQ LLAQSVNHGF ETVYELTKMC TIRMSFVKGW GAEYHRQDVT STPCWIEIHL HGPLQWLDKV LTQMGSPHNP ISSVS
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Smad1 is a critical intracellular signaling protein that plays a pivotal role in the Bone Morphogenetic Protein (BMP) signaling pathway, which is essential for various biological processes, including bone formation, cartilage development, and cell differentiation. The research surrounding recombinant Smad1 protein has gained momentum due to its implications in developmental biology and regenerative medicine. Understanding the functional mechanisms of Smad1 can provide insights into the regulatory networks that govern cellular responses to BMPs, which are vital for tissue engineering and therapeutic applications in bone diseases. Moreover, the ability to produce recombinant Smad1 protein allows researchers to conduct detailed biochemical studies and structure-function analyses, facilitating the identification of specific domains responsible for its activity and interactions with other proteins. This knowledge is crucial for developing novel strategies aimed at modulating BMP signaling, which could lead to innovative treatments for conditions such as osteoporosis and fractures. Consequently, the study of recombinant Smad1 not only enhances our understanding of fundamental biological processes but also opens avenues for potential clinical applications in enhancing bone regeneration and tissue repair.
