Analytical Data
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Gene name
CCNF
- Application
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Alternative Names
Ccnf; CCNF_HUMAN; Cyclin-F; F box only Protein 1; F-box only Protein 1; FBX1; FBXO1; G2 mitotic specific cyclin F; G2/mitotic specific cyclin F
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P41002
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Expression Region
1-786aa
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AA Sequence
MGSGGVVHCRCAKCFCYPTKRRIRRRPRNLTILSLPEDVLFHILKWLSVEDILAVRAVHSQLKDLVDNHASVWACASFQELWPSPGNLKLFERAAEKGNFEAAVKLGIAYLYNEGLSVSDEARAEVNGLKASRFFSLAERLNVGAAPFIWLFIRPPWSVSGSCCKAVVHESLRAECQLQRTHKASILHCLGRVLSLFEDEEKQQQAHDLFEEAAHQGCLTSSYLLWESDRRTDVSDPGRCLHSFRKLRDYAAKGCWEAQLSLAKACANANQLGLEVRASSEIVCQLFQASQAVSKQQVFSVQKGLNDTMRYILIDWLVEVATMKDFTSLCLHLTVECVDRYLRRRLVPRYRLQLLGIACMVICTRFISKEILTIREAVWLTDNTYKYEDLVRMMGEIVSALEGKIRVPTVVDYKEVLLTLVPVELRTQHLCSFLCELSLLHTSLSAYAPARLAAAALLLARLTHGQTQPWTTQLWDLTGFSYEDLIPCVLSLHKKCFHDDAPKDYRQVSLTAVKQRFEDKRYGEISQEEVLSYSQLCAALGVTQDSPDPPTFLSTGEIHAFLSSPSGRRTKRKRENSLQEDRGSFVTTPTAELSSQEETLLGSFLDWSLDCCSGYEGDQESEGEKEGDVTAPSGILDVTVVYLNPEQHCCQESSDEEACPEDKGPQDPQALALDTQIPATPGPKPLVRTSREPGKDVTTSGYSSVSTASPTSSVDGGLGALPQPTSVLSLDSDSHTQPCHHQARKSCLQCRPPSPPESSVPQQQVKRINLCIHSEEEDMNLGLVRL
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Molecular Weight
114 KDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CCNF, also known as Cyclin F, is a crucial protein that plays a significant role in cell cycle regulation and has garnered considerable attention in recent years due to its involvement in various cellular processes and diseases. As a member of the cyclin family, CCNF interacts with cyclin-dependent kinases (CDKs) to regulate the progression of cells through the cell cycle, particularly during the G2/M transition. Research has increasingly focused on the implications of CCNF dysregulation in cancer, where its altered expression can contribute to tumorigenesis and poor prognosis. Studies have demonstrated that CCNF is involved in regulating DNA repair mechanisms and maintaining genomic stability, highlighting its importance in preventing malignancies. Furthermore, recent findings have established that CCNF can modulate the stability and activity of several key oncogenic and tumor suppressor proteins, linking it to critical pathways in cancer biology. The exploration of CCNF as a potential therapeutic target is therefore profound, aiming to develop innovative strategies for cancer treatment. Additionally, CCNF's role in other biological processes, such as gametogenesis and stem cell differentiation, underscores its multifaceted functions. As researchers continue to dissect the molecular mechanisms underlying CCNF's actions within the cell, these investigations promise to shed light on new avenues for understanding cell cycle regulation and the development of cancer therapies. Understanding the intricate roles of CCNF not only contributes to basic biological knowledge but also holds potential for clinical applications in oncology and regenerative medicine.
