Analytical Data
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Gene name
SLC6A3
- Application
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Alternative Names
Sodium-dependent dopamine transporter. DA transporter. DAT. Solute carrier family 6 member 3
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q01959
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Expression Region
1-620 aa
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AA Sequence
MSKSKCSVGLMSSVVAPAKEPNAVGPKEVELILVKEQNGVQLTSSTLTNPRQSPVEAQDRETWGKKIDFLLSVIGFAVDLANVWRFPYLCYKNGGGAFLVPYLLFMVIAGMPLFYMELALGQFNREGAAGVWKICPILKGVGFTVILISLYVGFFYNVIIAWALHYLFSSFTTELPWIHCNNSWNSPNCSDAHPGDSSGDSSGLNDTFGTTPAAEYFERGVLHLHQSHGIDDLGPPRWQLTACLVLVIVLLYFSLWKGVKTSGKVVWITATMPYVVLTALLLRGVTLPGAIDGIRAYLSVDFYRLCEASVWIDAATQVCFSLGVGFGVLIAFSSYNKFTNNCYRDAIVTTSINSLTSFSSGFVVFSFLGYMAQKHSVPIGDVAKDGPGLIFIIYPEAIATLPLSSAWAVVFFIMLLTLGIDSAMGGMESVITGLIDEFQLLHRHRELFTLFIVLATFLLSLFCVTNGGIYVFTLLDHFAAGTSILFGVLIEAIGVAWFYGVGQFSDDIQQMTGQRPSLYWRLCWKLVSPCFLLFVVVVSIVTFRPPHYGAYIFPDWANALGWVIATSSMAMVPIYAAYKFCSLPGSFREKLAYAIAPEKDRELVDRGEVRQFTLRHWLKV
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Molecular Weight
94.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SLC6A3, also known as the dopamine transporter (DAT), plays a crucial role in the regulation of dopamine levels in the brain by facilitating the reuptake of dopamine from the synaptic cleft back into presynaptic neurons. Understanding the structure and function of SLC6A3 is vital, as dysregulation of dopamine signaling is implicated in various neuropsychiatric disorders such as schizophrenia, attention-deficit hyperactivity disorder (ADHD), and substance abuse. The study of recombinant SLC6A3 proteins allows researchers to investigate the transport mechanisms and pharmacological properties of DAT in controlled laboratory settings. This research is particularly important for drug development, as inhibiting or modulating DAT activity can lead to therapeutic strategies for conditions characterized by dopamine dysregulation. Techniques such as cryo-electron microscopy, X-ray crystallography, and functional assays using recombinant proteins have provided insights into the transporter’s structure-function relationship, including its binding sites and conformational changes during the transport cycle. Moreover, studying SLC6A3 through recombinant expression systems offers the possibility of exploring its interactions with various ligands, including psychostimulants and potential therapeutic agents, further elucidating its role in both normal physiology and disease states. The findings from such studies contribute significantly to our understanding of dopaminergic signaling and its broader implications in neuroscience and pharmacology.
