Analytical Data
-
基因名
CYP2R1
- Application
-
别名
CP2R1_HUMAN; CYP2R1; Cytochrome P450 2R1; Cytochrome P450 family 2 R1; Cytochrome P450 family 2 subfamily R polypeptide 1; EC 1.14.13.15; MGC4663; Vitamin D 25 hydroxylase; Vitamin D 25-hydroxylase
-
种属
Human
-
表达系统
E. coli
-
标签
GST-tag at N-terminal
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
Q6VVX0
-
表达区间
1-501aa
-
氨基酸序列
MWKLWRAEEGAAALGGALFLLLFALGVRQLLKQRRPMGFPPGPPGLPFIGNIYSLAASSELPHVYMRKQSQVYGEIFSLDLGGISTVVLNGYDVVKECLVHQSEIFADRPCLPLFMKMTKMGGLLNSRYGRGWVDHRRLAVNSFRYFGYGQKSFESKILEETKFFNDAIETYKGRPFDFKQLITNAVSNITNLIIFGERFTYEDTDFQHMIELFSENVELAASASVFLYNAFPWIGILPFGKHQQLFRNAAVVYDFLSRLIEKASVNRKPQLPQHFVDAYLDEMDQGKNDPSSTFSKENLIFSVGELIIAGTETTTNVLRWAILFMALYPNIQGQVQKEIDLIMGPNGKPSWDDKCKMPYTEAVLHEVLRFCNIVPLGIFHATSEDAVVRGYSIPKGTTVITNLYSVHFDEKYWRDPEVFHPERFLDSSGYFAKKEALVPFSLGRRHCLGEHLARMEMFLFFTALLQRFHLHFPHELVPDLKPRLGMTLQPQPYLICAERR
-
分子量
83.8 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Recombinant Human CLEC4C Protein，His
Cat：PA1000-7742
Recombinant Human VPS13A Protein，His
Cat：PA2000-3328
Protein Description
CYP2R1, a member of the cytochrome P450 superfamily, is primarily known for its role as a vitamin D 25-hydroxylase, which is crucial for the activation of vitamin D in the human body. This enzyme catalyzes the conversion of vitamin D3 (cholecalciferol) into 25-hydroxyvitamin D3 (calcidiol), the major circulating form of vitamin D that is essential for calcium homeostasis and bone health. Research has indicated that variations in the CYP2R1 gene may influence individual responses to vitamin D supplementation and impact overall health, including susceptibility to various diseases. Additionally, understanding the structural and functional properties of CYP2R1 through the study of its recombinant protein can provide insight into its enzymatic mechanisms and regulatory functions. This knowledge is crucial, as deficiencies or dysfunctions in vitamin D metabolism have been associated with conditions such as osteoporosis, autoimmune disorders, and certain cancers. Furthermore, the development of CYP2R1 recombinant protein serves as a valuable tool for drug discovery, enabling the identification of potential therapeutics that can modulate its activity. The study of CYP2R1 not only enhances our understanding of vitamin D metabolism but also opens avenues for research into personalized medicine approaches that can optimize vitamin D-related interventions for diverse populations.
