Analytical Data
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基因名
RPB5
- Application
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别名
RPB5；DNA-directed RNA polymerases I. II. and III subunit RPABC1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P19388
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表达区间
1-210aa
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氨基酸序列
MDDEEETYRL WKIRKTIMQL CHDRGYLVTQ DELDQTLEEF KAQSGDKPSE GRPRRTDLTV LVAHNDDPTD QMFVFFPEEP KVGIKTIKVY CQRMQEENIT RALIVVQQGM TPSAKQSLVD MAPKYILEQF LQQELLINIT EHELVPEHVV MTKEEVTELL ARYKLRENQL PRIQAGDPVA RYFGIKRGQV VKIIRPSETA GRYITYRLVQ
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分子量
24.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RPB5, a crucial subunit of RNA polymerase II, plays a significant role in the transcription of protein-coding genes by facilitating the assembly and stability of the polymerase complex. Research on RPB5 has been driven by its essential function in gene expression regulation and its involvement in various cellular processes. Abnormalities in RPB5 expression or function can lead to severe consequences, including developmental disorders and cancer. In recent years, studies have focused on the structural characteristics of RPB5, revealing its highly conserved nature across different species, suggesting its fundamental role in transcription mechanisms. Moreover, RPB5's interactions with other components of the transcription machinery highlight its importance in maintaining the fidelity of transcription and regulating gene expression in response to various environmental cues. By characterizing the properties of RPB5, researchers aim to uncover the intricate network of interactions that govern gene regulation, paving the way for potential therapeutic strategies targeting transcriptional dysregulation in disease contexts. Overall, the comprehensive understanding of RPB5 not only enhances our knowledge of fundamental biological processes but also opens up avenues for clinical applications in treating transcription-related pathologies.
