Analytical Data
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Gene name
ST8SIA5
- Application
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Alternative Names
ST8SIA5; SIAT8E; Alpha-2.8-sialyltransferase 8E; Sialyltransferase 8E; SIAT8-E; Sialyltransferase St8Sia V; ST8SiaV
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O15466
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Expression Region
1-376 aa
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AA Sequence
MRYADPSANRDLLGSRTLLFIFICAFALVTLLQQILYGRNYIKRYFEFYEGPFEYNSTRCLELRHEILEVKVLSMVKQSELFDRWKSLQMCKWAMNISEANQFKSTLSRCCNAPAFLFTTQKNTPLGTKLKYEVDTSGIYHINQEIFRMFPKDMPYYRSQFKKCAVVGNGGILKNSRCGREINSADFVFRCNLPPISEKYTMDVGVKTDVVTVNPSIITERFHKLEKWRRPFYRVLQVYENASVLLPAFYNTRNTDVSIRVKYVLDDFESPQAVYYFHPQYLVNVSRYWLSLGVRAKRISTGLILVTAALELCEEVHLFGFWAFPMNPSGLYITHHYYDNVKPRPGFHAMPSEIFNFLHLHSRGILRVHTGTCSCC
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Molecular Weight
70.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ST8SIA5, also known as polysialic acid (PSA) synthase, is an emerging focus in neuroscience and cancer research due to its crucial role in the synthesis of polysialic acid, a unique carbohydrate molecule that modulates cell-cell interactions and influences cellular behavior. Polysialic acid is primarily expressed on the surface of neurons and plays a significant role in neurodevelopment, synaptic plasticity, and neural regeneration. Alterations in ST8SIA5 expression and polysialic acid levels have been implicated in various neurological disorders, such as schizophrenia and autism, as well as in tumor progression and metastasis in cancer. The enzyme's ability to decorate cellular surfaces with polysialic acid facilitates neural cell migration and impacts immune responses, underscoring its potential as a therapeutic target. Recent studies have focused on elucidating the mechanisms underlying ST8SIA5 regulation and its interactions with other molecular pathways, aiming to develop novel strategies for intervention in both neurodegenerative diseases and cancer. Understanding the intricate dynamics of ST8SIA5 and its role in polysialic acid biosynthesis could pave the way for innovative treatments that leverage this pathway to enhance neural repair or inhibit tumor spread, highlighting its significance in both developmental biology and clinical applications.
